Hair follicle renewal:: organization of stem cells in the matrix and the role of stereotyped lineages and behaviors
Hair follicle renewal:: organization of stem cells in the matrix and the role of stereotyped lineages and behaviors
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DOI：
10.1242/dev.01975
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发表时间：
2005-09-01
期刊：
影响因子：
4.6
通讯作者：
Nicolas, JF
中科院分区：
文献类型：
--
作者：
Legué, E;Nicolas, JF
毛囊（HFs）通过位于毛囊隆突处的多能干细胞进行更新；然而，对于它们如何从增殖区（毛母质）产生多组织毛囊，我们知之甚少。为了解决这一问题，我们在毛囊生长过程中进行了暂时性的克隆标记诱导。与主流假说不同的是，我们发现毛母质中每种内部结构都含有受限的自我更新干细胞。这些细胞位于真皮乳头周围，形成生发层。它们占据不同的近远中区域，并通过定型的细胞谱系和细胞行为沿着毛母质的径向轴产生分化细胞。相比之下，毛囊的外层呈现出一种涉及细胞凋亡的生长模式，这种模式协调了内外层结构的发育。因此，毛囊形态是由沿着近远中轴的细胞命运组织以及毛母质中沿着径向（横向）轴的细胞行为所决定的。因此，我们的研究表明，命运和行为是由两个（非耦合的）系统所组织的，这种非耦合可能是简化形态发生的一种基本方式。
Hair follicles (HFs) are renewed via multipotent stem cells located in a reservoir (the bulge); however, little is known about how they generate multi-tissue HFs from a proliferative zone (the matrix). To address this issue, we temporally induced clonal labeling during HF growth. Challenging the prevailing hypothesis, we found that the matrix contains restricted self-renewing stem cells for each inner structure. These cells are located around the dermal papilla forming a germinative layer. They occupy different proximodistal sectors and produce differentiated cells along the matrix radial axis via stereotyped lineages and cell behavior. By contrast, the outer layer of HFs displays a mode of growth involving apoptosis that coordinates the development of outer and inner structures. HF morphology is therefore determined by the organization of cell fates along the proximodistal axis and by cell behavior along the radial (lateral) axis in the matrix. Thus, our studies suggest that fate and behavior are organized by two systems (uncoupled), and this uncoupling may represent a fundamental way to simplify morphogenesis.