Downregulation in aquaporin 4 and aquaporin 8 expression of the colon associated with the induction of allergic diarrhea in a mouse model of food allergy.
Downregulation in aquaporin 4 and aquaporin 8 expression of the colon associated with the induction of allergic diarrhea in a mouse model of food allergy.
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DOI：
10.1016/j.lfs.2007.04.036
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发表时间：
2007-06
期刊：
影响因子：
6.1
通讯作者：
Takeshi Yamamoto;H. Kuramoto;M. Kadowaki
中科院分区：
文献类型：
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作者：
Takeshi Yamamoto;H. Kuramoto;M. Kadowaki
在过去的几十年里，食物过敏变得越来越普遍。腹泻是由食物过敏原引起的最常见的肠道症状之一，其特点是离子交换和水转移不平衡；然而，过敏性腹泻的潜在机制仍不清楚。水穿过肠上皮膜的转移似乎是通过水通道蛋白（AQP）发生的。然而，水转运的分子机制和水通道蛋白在肠道中的病理生理作用尚未完全确定。目前的研究重点是过敏性腹泻小鼠模型中 AQP 的变化，其中 BALB/c 小鼠在反复口服卵清蛋白后出现腹泻。分别采用实时定量PCR分析和免疫组化技术检测AQP mRNA和蛋白的表达。与对照组相比，过敏小鼠近端结肠中的 AQP4 和 AQP8 mRNA 水平显着降低；同样，过敏小鼠近端结肠中 AQP4 和 AQP8 蛋白的表达也减少。这些结果表明过敏性腹泻与 AQP4 和 AQP8 表达下调相关。
Food allergies have become increasingly prevalent during the past few decades. Diarrhea is one of the most frequent intestinal symptoms caused by food allergens and is characterized by imbalanced ion exchange and water transfer; however, the underlying mechanism of allergic diarrhea remains unclear. Water transfer across the intestinal epithelial membrane seems to occur via aquaporins (AQPs). However, the molecular mechanism of water transfer and the pathophysiological roles of aquaporins in the intestine have not been fully established. The present studies have focused on the alterations of AQPs in a mouse model of allergic diarrhea in which BALB/c mice developed diarrhea following repeated challenges of orally administered ovalbumin. Quantitative real-time PCR analysis and immunohistochemical technique were used for expression of mRNA and protein of AQPs, respectively. AQP4 and AQP8 mRNA levels were significantly decreased in the proximal colon of allergic mice compared to controls; likewise, expression of AQP4 and AQP8 proteins was reduced in the proximal colon of the allergic mice. These results suggest that allergic diarrhea is associated with a downregulation in AQP4 and AQP8 expression.