Secretion pattern, ultrastructural localization and function of extracellular matrix molecules involved in eggshell formation
Secretion pattern, ultrastructural localization and function of extracellular matrix molecules involved in eggshell formation
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DOI：
10.1016/s0945-053x(00)00128-1
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发表时间：
2001-01-01
期刊：
影响因子：
6.9
通讯作者：
Arias, JL
中科院分区：
文献类型：
--
作者：
Fernandez, MS;Moya, A;Arias, JL
鸡蛋壳是一种含有有机相和无机相的复合生物陶瓷。有机相含有X型胶原和蛋白聚糖（乳头蛋白聚糖，硫酸角质素蛋白聚糖，和卵聚糖，硫酸皮肤素蛋白聚糖），其定位取决于地形定义和时间调节的沉积。虽然这些大分子在蛋壳中的分布已经得到很好的确定，但对它们在蛋壳亚结构和输卵管细胞中的精确定位或它们在蛋壳形成过程中的生产和功能模式知之甚少。通过免疫荧光和免疫电镜分析，我们研究了这些大分子在输卵管细胞中的分布在不同的产卵后时间。为了了解蛋白多糖硫酸化对蛋壳形成的作用，我们研究了氯酸钠处理对蛋白多糖硫酸化的抑制作用。我们发现，这些大分子是由特定的输卵管细胞群，并在精确的产卵后时间。基于这些大分子在蛋壳亚结构中的精确超微结构定位、输卵管细胞分泌这些大分子的时间以及蛋白聚糖硫酸化抑制对蛋壳形成的影响，推测乳头蛋白聚糖的作用是在第一个方解石晶体的成核中，而卵聚糖的作用是调节后来形成的蛋壳晶体的生长和取向。(C)2001 Elsevier Science B.V./国际矩阵生物学学会。All rights reserved.
The chicken eggshell is a composite bioceramic containing organic and inorganic phases. The organic phase contains, among other constituents, type X collagen and proteoglycans (mammillan, a keratan sulfate proteoglycan, and ovoglycan, a dermatan sulfate proteoglycan), whose localization depends on a topographically defined and temporally regulated deposition. Although the distribution of these macromolecules in the eggshell has been well established, little is known about their precise localization within eggshell substructures and oviduct cells or their pattern of production and function during eggshell formation. By using immunofluorescent and immuno-ultrastructural analyses, we examined the distribution of these macromolecules in oviduct cells at different post-oviposition times. To understand the role of proteoglycan sulfation on eggshell formation, we studied the effects of inhibition of proteoglycan sulfation by treatment with sodium chlorate. We showed that these macromolecules are produced by particular oviduct cell populations and at precise post-oviposition times. Based on the precise ultrastructural localization of these macromolecules in eggshell substructures, the timing of the secretion of these macromolecules by oviduct cells and the effects on eggshell formation caused by the inhibition of proteoglycan sulfation, the putative role of mammillan is in the nucleation of the first calcite crystals, while that of ovoglycan is to regulate the growth and orientation of the later forming crystals of the chicken eggshell. (C) 2001 Elsevier Science B.V./International Society of Matrix Biology. All rights reserved.