Use of Atomic Force Microscopy in UVB-Induced Chromosome Damage Provides Important Bioinformation for Cell Damage Assessment.
Use of Atomic Force Microscopy in UVB-Induced Chromosome Damage Provides Important Bioinformation for Cell Damage Assessment.
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DOI：
10.1021/acs.langmuir.3c01644
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发表时间：
2023-09
期刊：
影响因子：
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通讯作者：
Bowei Wang;Jianjun Dong;Fan Yang;Tuoyu Ju;Jiani Li;Junxiang Wang;Ying Wang;M. J. C. Crabbe;Yuandong Tian;Zuobin Wang
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文献类型：
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作者：
Bowei Wang;Jianjun Dong;Fan Yang;Tuoyu Ju;Jiani Li;Junxiang Wang;Ying Wang;M. J. C. Crabbe;Yuandong Tian;Zuobin Wang
用原子力显微镜(AFM)观察UVB辐射对HaCaT细胞染色体结构的影响。结果表明，UVB辐射剂量越大，染色体畸变率越高。同时对具有代表性的不同类型的染色体结构异常进行了研究。我们还发现，在相应的辐射剂量下，DNA和细胞都会受到损伤。研究发现，DNA损伤程度与辐射剂量成正比。UVB照射后细胞的力学性质也发生了变化，提示细胞在照射后经历了从内到外的一系列连锁反应。UVB辐射后AFM对染色体结构的高分辨率成像使我们能够将UVB辐射引起的染色体、DNA和细胞之间的损伤联系起来，并提供关于遗传效应的特定信息。
The chromosomal structure derived from UVB-stimulated HaCaT cells was detected by atomic force microscopy (AFM) to evaluate the effect of UVB irradiation. The results showed that the higher the UVB irradiation dose, the more the cells that had chromosome aberration. At the same time, different representative types of chromosome structural aberrations were investigated. We also revealed damage to both DNA and cells under the corresponding irradiation doses. It was found that the degree of DNA damage was directly proportional to the irradiation dose. The mechanical properties of cells were also changed after UVB irradiation, suggesting that cells experienced a series of chain reactions from inside to outside after irradiation. The high-resolution imaging of chromosome structures by AFM after UVB irradiation enables us to relate the damage between chromosomes, DNA, and cells caused by UVB irradiation and provides specific information on genetic effects.