Marker-assisted backcrossing for simultaneous introgression of two genes
Marker-assisted backcrossing for simultaneous introgression of two genes
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DOI：
10.2135/cropsci2001.1716
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发表时间：
2001-11-01
期刊：
影响因子：
2.3
通讯作者：
Melchinger, AE
中科院分区：
文献类型：
--
作者：
Frisch, M;Melchinger, AE
标记辅助选择用于在回交程序中加速轮回亲本基因组（RPG）的恢复。我们的目标是比较各种选择策略和育种计划的同时渗入两个基因的RPG回收和所需的标记数据点（MDP）的数量。利用已发表的玉米（Zea mays L.）由80个标记组成，并假设基于表型评价选择显性靶基因。对于非连锁以及连锁的目标基因，backeross程序从六代缩短到三代，导致应用标记辅助背景选择。在具有三个回交世代的育种程序中，当（i）应用由基于目标基因的存在和从标记基因型计算的选择指数的三个或四个选择步骤组成的选择策略，（ii）从早代到高代增加群体大小，以及（iii）在早代中合并目标基因时，需要最少的MDP。这些原则可用于优化设计的标记辅助回交程序的同时渗入的两个基因。
Marker-assisted selection is used to accelerate recovery of the recurrent parent genome (RPG) in backcross programs. Our objectives were to compare various selection strategies and breeding plans for the simultaneous introgression of two genes with respect to the RPG recovered and the number of marker data points (MDP) required. Computer simulations were performed with a published genetic map in maize (Zea mays L.) consisting of 80 markers and assuming selection for dominant target genes on the basis of phenotypic evaluation. For unlinked as well as for linked target genes, a shortening of the backeross program from six to three generations resulted from applying marker-assisted background selection. In breeding programs with three backcross generations, the least MDP were required when (i) applying selection strategies consisting of three or four selection steps on the basis of presence of the target genes and selection indices calculated from the marker genotype, (ii) increasing the population size from early to advanced generations, and (iii) merging the target genes in an early generation. These principles can be used for optimizing the design of marker-assisted backcross programs for the simultaneous introgression of two genes.