ANTIBODIES OF RESTRICTED HETEROGENEITY OBTAINED IN RABBITS IMMUNIZED WITH TETRAGLYCINE PEPTIDE CONJUGATED TO AN AUTOLOGOUS CARRIER
ANTIBODIES OF RESTRICTED HETEROGENEITY OBTAINED IN RABBITS IMMUNIZED WITH TETRAGLYCINE PEPTIDE CONJUGATED TO AN AUTOLOGOUS CARRIER
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DOI：
10.1002/eji.1830041205
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发表时间：
1974-01-01
影响因子：
5.4
通讯作者：
ESCRIBANO, MJ
中科院分区：
文献类型：
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作者：
ESCRIBANO, MJ
已在用连接至自体载体的肽免疫的兔中获得对四肽Gly-Gly-Gly-Gly的特异性抗体。使用戊二醛进行结合。二次免疫后，部分家兔产生抗四肽抗体，用四肽-琼脂糖免疫吸附剂从血清中分离出抗四肽抗体。所获得的量在20至200 μg/ml血清之间变化。发现分离的特异性抗四肽抗体属于IgG和IgM类别。通过等电聚焦分析的IgG抗体显示出非常有限的pI谱。IgG和IgM抗体均表现出有限的同种异型特异性。
Antibodies specific for the tetrapeptide Gly‐Gly‐Gly‐Gly have been obtained in rabbits immunized with the peptide linked to an autologous carrier. Conjugation was performed using glutaraldehyde. Peptide‐carrier conjugates contained only one or two molecules of peptide per carrier molecule.After secondary immunization, some of the rabbits produced anti‐tetrapeptide antibodies which were isolated from individual sera using tetrapeptide‐Sepharose immunoadsorbent. The amount obtained varied from 20 to 200 μg/ml of serum. Isolated specific anti‐tetrapeptide antibodies were found to belong to both IgG and IgM classes. The IgG antibodies as analyzed by isoelectric focusing showed a very restricted pI spectrum. Both IgG and IgM antibodies showed a restricted allotypic specificity.