Visual and Motor Deficits in Grown-up Mice with Congenital Zika Virus Infection.
Visual and Motor Deficits in Grown-up Mice with Congenital Zika Virus Infection.
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DOI：
10.1016/j.ebiom.2017.04.029
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发表时间：
2017-06
期刊：
影响因子：
11.1
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Cui L;Zou P;Chen E;Yao H;Zheng H;Wang Q;Zhu JN;Jiang S;Lu L;Zhang J
患有先天性寨卡病毒（ZIKV）感染的人类婴儿表现出一系列症状，包括小头畸形、颅内钙化、黄斑萎缩和关节弯曲。更重要的是，预后数据远远落后于2015年最近爆发的ZIKV。在这项工作中，我们让先天感染ZIKV的小鼠发育到青春期。这些小鼠表现出运动不协调和视觉功能障碍，这可以通过视网膜和小脑皮质的解剖缺陷来解释。相反，ZIKV感染小鼠的焦虑水平是正常的。不同小鼠的解剖学和行为缺陷谱是一致的。我们的数据提供的证据可能有助于预测动物模型中ZIKV相关先天性脑畸形预后方面的公共卫生负担。我们的研究为先天性ZIKV感染的预后提供了行为评估，并为筛选和评估候选药物和治疗提供了平台，旨在改善受感染神经元的再生，以预防胎儿ZIKV感染引起的后遗症。具有先天性ZIKV感染的小鼠在青春期表现出运动缺陷，可能导致瘫痪。患有先天性ZIKV感染的小鼠具有未分层的视网膜和扭曲的视觉。该研究具有人先天性ZIKV感染的预后意义。最近寨卡病毒的爆发以及随之而来的出生缺陷婴儿数量的增加引起了人们的极大关注。然而，关于这些先天性异常儿童的预后知识却相对滞后。在这项研究中，我们将先天感染ZIKV的小鼠培养到青春期。我们的研究揭示了受感染小鼠明显的运动和视觉缺陷以及颅内钙化，这可能有助于预测公共卫生负担。
Human infants with congenital Zika virus (ZIKV) infection exhibit a range of symptoms including microcephaly, intracranial calcifications, macular atrophy and arthrogryposis. More importantly, prognosis data have lagged far behind the recent outbreak of ZIKV in 2015. In this work, we allow congenitally ZIKV-infected mice to grow into puberty. These mice exhibited motor incoordination and visual dysfunctions, which can be accounted by anatomical defects in the retina and cerebellar cortex. In contrary, anxiety level of the ZIKV-infected mice is normal. The spectrum of anatomical and behavioral deficits is consistent across different mice. Our data provided evidence that may help predict the public health burden in terms of prognosis of ZIKV-related congenital brain malformations in an animal model. Our study provided behavioral evaluation for the prognosis of congenital ZIKV infection and provides a platform for screening and evaluation of drugs candidates and treatment aiming at improving regeneration of infected neurons to prevent sequelae caused by ZIKV infection of fetus. Mice with congenital ZIKV infection manifest motor deficits in puberty may lead to paralysis. Mice with congenital ZIKV infection have unlaminated retina and distorted vision. This study has prognostic implication of human congenital ZIKV infection. The recent outbreak of Zika virus and consequent increased number of babies with birth defects has raised great concern. However, knowledge about the prognosis of these children with congenital abnormalities has lagged behind. In this study, we raised congenitally ZIKV-infected mice into puberty. Our study revealed prominent motor and visual deficits as well as intracranial calcifications in the infected mice, which may help predict the public health burden.