Cryptotanshinone downregulates the profibrotic activities of hypertrophic scar fibroblasts and accelerates wound healing: A potential therapy for the reduction of skin scarring
Cryptotanshinone downregulates the profibrotic activities of hypertrophic scar fibroblasts and accelerates wound healing: A potential therapy for the reduction of skin scarring
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隐丹参酮下调肥厚性疤痕成纤维细胞的促纤维化活性并加速伤口愈合：减少皮肤疤痕的潜在疗法
DOI：
10.1016/j.biopha.2016.03.006
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发表时间：
2016-05-01
影响因子：
7.5
通讯作者：
Hu, Dahai
中科院分区：
文献类型：
--
作者：
Li, Yan;Shi, Shan;Hu, Dahai
增生性瘢痕（HS）是一种引起主要临床问题症状的皮肤纤维化疾病。隐丹参酮（Cryptotanshinone，CT）是丹参（Salvia miltiorrhiza Bunge extract）的重要成分，已被用于治疗心脑血管疾病。其在HS中的临床疗效尚不清楚。为了研究CT是否可以抑制HS纤维化，建立HS衍生的成纤维细胞（HSF），并用或不用CT处理。通过Western blot和实时定量聚合酶链反应测定I型胶原（Col 1）、III型胶原（Col 3）和α-平滑肌肌动蛋白（α-SMA）的表达。分别用划痕试验和成纤维细胞聚集胶原晶格（FPCL）收缩试验评估HSFs迁移和收缩。通过胶原蛋白表达的免疫组织化学分析和胶原蛋白沉积的Masson三色染色分析来评估CT处理的Balb/c小鼠中的伤口愈合。CT处理HSFs下调Col 1、Col 3和α-SMA mRNA和蛋白表达，HSFs迁移和HSFs收缩，并改善FPCL结构。在小鼠中，CT治疗加速了伤口愈合：疤痕边缘变窄，再生组织中的胶原蛋白沉积减少。因此，CT促进伤口愈合并减少细胞外基质成分的过度沉积。CT可以帮助预防和减少疤痕。(C)2016 Elsevier Masson SAS。All rights reserved.
Hypertrophic scar (HS) is a skin fibrotic disease that causes major clinically problematic symptoms. Cryptotanshinone (CT) is an important ingredient of Danshen (Salvia miltiorrhiza Bunge extract) that has been used to treat cardio-cerebral vascular diseases. Its clinical efficacy in HS remains unclear. To investigate whether CT can inhibit HS fibrosis, HS-derived fibroblastic cells (HSFs) were established and treated with or without CT. Type-collagen-I (Col1), type-collagen-III (Col3) and alpha-smooth muscle actin (alpha-SMA) expression were measured by western blot and real-time quantitative polymerase chain reaction. HSFs migration and contraction were assessed with the scratch assay and the fibroblast-populated collagen lattice (FPCL) contraction assay, respectively. Wound healing in CT-treated Balb/c mice was assessed by immunohistochemical analysis of collagen expression and Masson's trichrome staining analysis of collagen deposition. CT treatment of HSFs down-regulated Col1, Col3 and alpha-SMA mRNA and protein expression, HSFs migration, and HSFs contraction, and improved FPCL architecture. In mice, CT treatment accelerated wound healing: the scar margins were narrow and there was less collagen deposition in the regenerated tissue. Thus, CT promotes wound healing and decreases excessive deposition of extracellular matrix components. CT may help to prevent and reduce scarring. (C) 2016 Elsevier Masson SAS. All rights reserved.