Micromanipulation measurements of biological materials
Micromanipulation measurements of biological materials
复制标题
生物材料的显微操作测量
DOI：
10.1023/a:1005644412588
复制
发表时间：
2000
影响因子：
2.7
通讯作者：
C. Cowen
中科院分区：
文献类型：
--
作者：
Colin R. Thomas;Zhibing Zhang;C. Cowen
显微操作可以在特定的细胞中测量微观生物颗粒的机械特性。它能够测量高变形，包括细胞破裂。颗粒小至1 μm，破断力低至1 μN。该方法可以通过力学建模来增强，以允许估计细胞壁弹性模量等内在力学特性。目前和潜在的应用包括研究酵母和细菌细胞的破坏，悬浮培养中动物细胞的机械损伤和水中发酵中的丝状微生物，食品加工中的植物细胞行为以及絮凝过程。
Micromanipulation enables the mechanical properties of microscopic biological particles to be measured in particular cells. It is capable of measurements at high deformations, including up to cell bursting. Particles as small as 1 μm, with breaking forces as low as 1 μN, can be characterised. The method can be enhanced by mechanical modelling to allow intrinsic mechanical properties such as the cell wall elastic modulus to be estimated. Present and potential applications include studying yeast and bacterial cell disruption, mechanical damage to animal cells in suspension cultures and filamentous microorganisms in submerged fermentations, plant cell behaviour in food processing, and flocculation processes.
影响因子：
2.4
作者：
Jones, WR;Ting-Beall, HP;Guilak, F
通讯作者：
Guilak, F