NK-lysin, structure and function of a novel effector molecule of porcine T and NK cells
NK-lysin, structure and function of a novel effector molecule of porcine T and NK cells
复制标题
DOI：
10.1016/s0165-2427(96)05677-2
复制
发表时间：
1996-11-01
影响因子：
1.8
通讯作者：
Gudmundsson, GH
中科院分区：
文献类型：
--
作者：
Andersson, M;Gunne, H;Gudmundsson, GH
NK-赖氨酸(NKL)是从猪小肠中分离得到的一种78个氨基酸残基的抗菌肽。标准方法鉴定该肽是碱性的，在三个链内二硫键上有六个半胱氨酸残基。该序列与激活的T细胞和NK细胞的部分基因产物(NKG5)有33%的同源性。NK-Lysin对大肠埃希菌和巨大芽孢杆菌有明显的抗菌活性，对NK敏感的肿瘤细胞株YAC-I有明显的杀伤活性，而对绵羊红细胞无明显影响。我们还分析了该基因在细胞和组织中的特异性表达和诱导。在人白介素2(IL-2)的刺激下，富含T和NK细胞的淋巴细胞部分显示NKL转录本增加30倍。在脾、骨髓和结肠中也可检测到NK-溶素特异性的mRNA。免疫组织化学染色显示NKL存在于不同类型的淋巴细胞中。我们的结果有力地表明，NK-溶素参与了T和NK细胞的诱导细胞毒作用。
NK-lysin (NKL), a 78-residue antimicrobial peptide, was isolated from pig small intestine. Standard methods identified the peptide as basic, with six half-cystine residues in three intrachain disulphide bonds. The sequence showed 33% identity with a part of a putative gene product (NKG5) from activated T and NK cells. NK-lysin showed antibacterial activity against Escherichia coli and Bacillus megaterium and marked lytic activity against YAC-I, a NK sensitive tumour cell line, while sheep red blood cells were unaffected.The cDNA clone corresponding to NK-lysin has been characterized. We have also analyzed the cell and tissue specific expression and the induction of the gene. A lymphocyte fraction enriched in T and NK cells, stimulated by human interleukin-2 (IL-2), showed a 30-fold increase of the NKL transcript. NK-lysin specific mRNA is also detectable in spleen, bone marrow and colon. Immunostaining showed NKL to be present in different types of lymphocytes. Our results strongly suggest that NK-lysin is involved in the inducible cytotoxicity of T and NK cells.