Accelerated solvent extraction of phenolic compounds exploiting a Box-Behnken design and quantification of five flavonoids by HPLC-DAD in Passiflora species
Accelerated solvent extraction of phenolic compounds exploiting a Box-Behnken design and quantification of five flavonoids by HPLC-DAD in Passiflora species
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DOI：
10.1016/j.microc.2016.12.021
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发表时间：
2017-05-01
影响因子：
4.8
通讯作者：
David, Jorge M.
中科院分区：
文献类型：
--
作者：
Gomes, Silvana V. F.;Portugal, Lindomar A.;David, Jorge M.
本工作描述了一种方法的开发，用于提取酚类化合物，主要是黄酮类化合物，从西番莲属物种，采用加速溶剂萃取（ASE）和使用Box-Behnken设计与期望函数进行优化。最佳提取条件为：提取温度80 ℃、乙醇浓度64%、提取次数5次。采用高效液相色谱-二极管阵列检测器（HPLC-DAD）对西番莲叶中的5种黄酮类化合物进行定性和定量分析。还开发并验证了使用乙腈（溶剂B）和0.2%（w/w）甲酸水溶液（溶剂A）作为洗脱液混合物的梯度洗脱。这五种药物的定量具有良好的线性、LOQ、LOD、精密度和准确度。结果表明，在紫丁香中含有较高浓度的异牡荆素、牡荆素、异牡荆素和芦丁。黄果（1.61 mg/g提取物和0.58 mg/g干植物），桑叶（2.10 mg/g提取物和0.90 mg/g干植物），刚毛假单胞菌（2.48 mg/g提取物和0.97 mg/g干植物）、刚毛假单胞菌（8.46 mg/g提取物和3.30 mg/g干植物）和球斑紫菜（3.48 mg/g提取物和1.02 mg/g干植物）。(C)2017 Elsevier B. V.保留所有紧身衣。
This work describes the development of a method for the extraction of phenolic compounds, mainly fiavonoids, from species of Passiflora by employing accelerated solvent extraction (ASE) and using a Box-Behnken design with desirability functions for optimization. The optimal extraction conditions consisted of an extraction temperature of 80 degrees C, 64% (w/w) ethanol and five number of extraction cycles. A high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of orientin, isoorientin, vitexin, isovitexin and rutin flavonoids in the leaves of seventeen Passiflora spp. using gradient elution with acetonitrile (solvent B) and 0.2% (w/w) formic acid in water (solvent A) as the eluent mixture was also developed and validated. These five fiavonoids were quantified with good linearity, LOQs, LODs, precision, and accuracy. Higher concentrations of isoorientin, orientin, vitexin, isovitexin and rutin were determinate in P. edulis f. flavicarpa (1.61 mg/g extract and 0.58 mg/g dry plant), P. morifolia (2.10 mg/g extract and 0.90 mg/g dry plant), P. setacea (2.48 mg/g extract and 0.97 mg/g dry plant), P. setacea (8.46 mg/g extract and 3.30 mg/g dry plant) and P. galbana (3.48 mg/g extract and 1.02 mg/g dry plant), respectively. (C) 2017 Elsevier B.V. All tights reserved.