Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish
Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish
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DOI：
10.1016/j.bone.2011.02.004
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发表时间：
2011-05-01
期刊：
影响因子：
4.1
通讯作者：
Martin, T. John
中科院分区：
文献类型：
--
作者：
Suzuki, Nobuo;Danks, Janine A.;Martin, T. John
采用硬骨鱼鳞片和成骨细胞碱性磷酸酶（ALP）和破骨细胞抗酒石酸酸性磷酸酶（TRAP）指标，研究了河豚PTH 1对硬骨鱼成骨细胞和破骨细胞的影响。合成的河豚PTH 1（1-34）（100 pg/m1-10 ng/ml）在孵育6 h时显著增加ALP活性。高剂量（10 ng/ml）河豚PTH 1显着增加ALP活性，即使在18小时的孵育。在TRAP活性的情况下，河豚PTH 1在孵育6 h时没有变化，但在18 h时，河豚PTH 1（100 pg/m1-10 ng/ml）显著增加TRAP活性。对人PTH（1-34）获得了类似的结果，但对河豚PTH 1的反应比对人PTH的反应更大。在体外实验中，我们证实了河豚PTH 1处理后，成骨细胞中NF-κ B配体的受体激活剂和破骨细胞中NF-κ B mRNA的表达均显著增加。在体内实验中，河豚PTH 1诱导的高钙血症是由于注射后鳞片中成骨细胞和骨细胞活性增加以及鳞片钙含量降低所致。此外，肌内自体移植鳞片的体外实验表明，PTH处理的鳞片中的多核破骨细胞/单核破骨细胞的比率显著高于对照鳞片。因此，我们认为PTH作用于鳞片中的成骨细胞和破骨细胞，并调节金鱼的钙代谢。(C)2011 Elsevier Inc. All rights reserved.
The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100 pg/m1-10 ng/ml) significantly increased ALP activity at 6 h of incubation. High-dose (10 ng/ml) fugu PTH1 significantly increased ALP activity even after 18 h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6 h of incubation, but fugu PTH1 (100 pg/m1-10 ng/ml) significantly increased TRAP activity at 18 h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-kappa B ligand in osteoblasts and the receptor activator NF-kappa B mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish. (C) 2011 Elsevier Inc. All rights reserved.