Time sequence of germinal vesicle breakdown in pig oocytes after cycloheximide and P-aminobenzamidine block.
Time sequence of germinal vesicle breakdown in pig oocytes after cycloheximide and P-aminobenzamidine block.
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放线菌酮和对氨基苯甲脒阻断后猪卵母细胞生发囊泡破裂的时间序列。
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发表时间：
1988
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通讯作者：
J. Fulka
中科院分区：
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作者：
M. Kubelka;J. Motík;J. Fulka;R. Procházka;Z. Rimkevičová;J. Fulka
猪卵母细胞在含10 μ g/ml放线菌酮的培养液中培养20小时后，均处于生殖泡期，但核质中的二价体高度浓缩。当这些卵母细胞洗涤和培养在对照培养基中为2，4，和6小时，生殖囊泡破裂（GVBD）完成，分别为0，86，和100%。当同样的抑制卵母细胞培养连续只有2.5小时的对照培养基再次给予放线菌酮富集培养基（3.5小时），几乎所有的人再次达到终变期晚期。这意味着培养20小时并洗涤不含这种蛋白质合成抑制剂的卵母细胞快速完成GVBD（4小时），并且在洗涤抑制剂后的前2小时内已经发生了对核膜崩解至关重要的蛋白质合成。所有的卵母细胞培养20小时，在培养基中与1 mM的对氨基苯甲脒休息在GV与染色质周围的紧凑的核仁。放线菌酮（20小时）和对氨基苯甲脒（10小时）的连续培养防止GVBD在所有的卵母细胞，太。相比之下，当将环己酰亚胺封闭（20小时）后清洗的卵母细胞在富含对氨基苯甲脒的培养基中培养2和3小时，并在环己酰亚胺培养基中再次培养6小时时，核膜分别溶解在62%和68%的卵母细胞中。这些数据表明，抑制猪卵母细胞中的蛋白质合成并不能阻止GV中二价体的高度浓缩。然而，核膜的破坏需要连续的蛋白质合成和蛋白质水解。
All porcine oocytes cultured 20 hr in medium with 10 micrograms/ml cycloheximide rested in the germinal vesicle (GV) stage but with the highly condensed bivalents in nucleoplasm. When these oocytes were washed and cultured in the control medium for 2, 4, and 6 hr, germinal vesicle breakdown (GVBD) was completed in 0, 86, and 100% of them, respectively. When similarly inhibited oocytes cultured successively only 2.5 hr in the control medium were given again in cycloheximide enriched medium (3.5 hr), nearly all of them reached late diakinesis stage again. It means that oocytes cultured for 20 hr and washed free of this inhibitor of protein synthesis completed GVBD rapidly (4 hr) and protein synthesis crucial for nuclear membrane disintegration occurred already during the first 2 hr after washing of inhibitor. All oocytes cultured for 20 hr in medium with 1 mM p-aminobenzamidine rested in GV with chromatin around the compact nucleolus. The successive culture in cycloheximide (20 hr) and p-aminobenzamidine (10 hr) prevented GVBD in all oocytes, too. In contrast, when the oocytes washed after cycloheximide block (20 hr) were cultured in p-aminobenzamidine enriched medium 2 and 3 hr and again for 6 hr in cycloheximide medium, the nuclear membrane dissolved in 62 and 68% of oocytes, respectively. These data suggest that inhibition of protein synthesis in pig oocytes does not prevent the high condensation of bivalents in GV. However, nuclear membrane breakdown requires the successive protein synthesis and proteolysis.