Glycoglycerolipid from the membranes of Acholeplasma laidlawii binds to human immunodeficiency virus-1 (HIV-1) and accelerates its entry into cells
Glycoglycerolipid from the membranes of Acholeplasma laidlawii binds to human immunodeficiency virus-1 (HIV-1) and accelerates its entry into cells
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DOI：
10.1007/s00284-003-4101-x
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发表时间：
2004-03-01
影响因子：
2.6
通讯作者：
Kuwano, K
中科院分区：
文献类型：
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作者：
Shimizu, T;Arai, S;Kuwano, K
我们以前报道过，从laidlawii的膜中提取的糖甘油脂，3-O-[2'- o -（α -d -葡萄糖吡喃基）-6'- o -酰基- α -葡萄糖吡喃基]1-1,2-二- o -酰基- sng -甘油（gagdg）与人类细胞系结合。此外，在人类细胞系中发现gagdg增加HIV-1感染。我们发现GAGDG与HIV-1结合并促进HIV-1进入细胞。抗GAGDG血清阻断了GAGDG与HIV-1的结合能力。与合成的gagdg和相关化合物的结合实验表明，在C14或C16位置上存在分支形式的酰基链，葡萄糖和与葡萄糖结合的酰基链是有效结合的关键。在单周期复制实验中，GAGDG有效地增强了HIV-1进入细胞的能力。这些结果表明，瓢虫膜的GAGDG参与了促进HIV-1感染的过程。
We have reported previously that glycoglycerolipids derived from the membranes of Acholeplasma laidlawii, 3-O-[2'-O-(alpha-D-glucopyranosyl)-6'-O-acyl-alpha-glucopyranosy]1-1,2-di-O-acyl-sn-glycerols (GAGDGs) bind to human cell lines. In addition, the GAGDGs were found to augment HIV-1 infection in human cell lines. Here we show that GAGDG binds to HIV-1 and facilitates the entry of HIV-1 into cells. The binding ability of GAGDG to HIV-1 was blocked by anti-GAGDG serum. Binding assay with synthetic GAGDGs and related compounds showed that the presence of branching form of acyl chains at the C14 or C16 position, glucose, and the acyl chain binding to the glucose were critical for efficient binding. GAGDG efficiently augmented the entry of HIV-1 into cells in a single-cycle replication assay. These results indicate that GAGDG of A. laidlawii membranes participates in the facilitation of HIV-1 infection.