Biofilm microbial communities of denture stomatitis
Biofilm microbial communities of denture stomatitis
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DOI：
10.1111/j.1399-302x.2008.00445.x
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发表时间：
2008-10-01
影响因子：
--
通讯作者：
Nobrega, F. G.
中科院分区：
文献类型：
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作者：
Campos, M. S.;Marchini, L.;Nobrega, F. G.
简介： 假牙口腔炎是影响假牙佩戴者的常见病变。其多因素病因似乎取决于复杂且特征不明的生物膜。本研究的目的是使用不依赖培养的方法评估患有和不患有义齿口腔炎的全口义齿佩戴者获得的微生物生物膜的组成。方法：从健康的义齿佩戴者和患有义齿口腔炎的患者中收集样本。对通过聚合酶链式反应获得的包含约 600 个克隆的 16S 核糖体 DNA (rDNA) 细菌序列和 192 个克隆的真核内转录间隔区 (ITS) 区域序列的文库进行了分析。结果：部分 16S rDNA 序列揭示了在健康受试者和义齿性口炎患者中鉴定出的总共 82 个细菌种类。两种生物膜中均检测到 27 种细菌，其中 29 种仅存在于义齿性口炎患者中，26 种仅存在于健康受试者中。对 ITS 区域的分析揭示了念珠菌的存在。结论：结果揭示了微生物菌群的范围，表明健康受试者和义齿口腔炎患者中存在不同的生物膜。
Introduction: Denture stomatitis is a common lesion that affects denture wearers. Its multifactorial etiology seems to depend on a complex and poorly characterized biofilm. The purpose of this study was to assess the composition of the microbial biofilm obtained from complete denture wearers with and without denture stomatitis using culture-independent methods.Methods: Samples were collected from healthy denture wearers and from patients with denture stomatitis. Libraries comprising about 600 cloned 16S ribosomal DNA (rDNA) bacterial sequences and 192 cloned eukaryotic internal transcribed spacer (ITS) region sequences, obtained by polymerase chain reactions, were analyzed.Results: The partial 16S rDNA sequences revealed a total of 82 bacterial species identified in healthy subjects and patients with denture stomatitis. Twenty-seven bacterial species were detected in both biofilms, 29 species were exclusively present in patients with denture stomatitis, and 26 were found only in healthy subjects. Analysis of the ITS region revealed the presence of Candida sp. in both biofilms.Conclusion: The results revealed the extent of the microbial flora, suggesting the existence of distinct biofilms in healthy subjects and in patients with denture stomatitis.