In vivo Studies on the Occurrence of Stored mRNA in Embryonic Axes of Vigna unguiculata Seeds
In vivo Studies on the Occurrence of Stored mRNA in Embryonic Axes of Vigna unguiculata Seeds
复制标题
荠菜种子胚轴储存 mRNA 发生的体内研究
DOI：
10.1093/oxfordjournals.pcp.a076657
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
T. Minamikawa
中科院分区：
文献类型：
--
作者：
Yoshiharu Suzuki;T. Minamikawa
测定了不同浓度的α-Amanitin和虫草素对钩吻豆胚轴开始吸胀后鲜重增加的抑制作用，以及对36-38h胚轴上~3H标记亮氨酸参入率的影响。α-Amanitin在0.5~5μ/kg/ml范围内对鲜重增长和蛋白质合成均有明显的抑制作用。当药物浓度为1μg/ml时，对吸胀早期(4h)的蛋白质合成无明显影响，而放线菌酮是一种非常有效的抑制剂。通过在α-Amanitin存在下，允许“干”轴吸食~3H-腺苷溶液4和12h的实验，发现早在开始吸食4h后，轴心就有不同程度的新合成，药物能有效地抑制Poly A+RNA的合成。这一结果可能表明在V胚轴中存在储存的mRNA。[医]蜗牛结籽。
α-Amanitin and cordycepin at various concentrations were tested for their inhibitory effect on the fresh weight increase ofVigna unguiculataembryonic axes after the onset of imbibition and on the incorporation rate of3H-labeled leucine into protein in axes of the 36–38 h stage. α-Amanitin at 0.5–5 μ/Kg/ml clearly exerted an inhibitory effect on both the fresh weight increase and the protein synthesis. This drug at 1 μg/ml, however, showed no significant effect on the protein synthesis at an early stage of imbibition (4 h), whereas cycloheximide was a very potent inhibitor. By experiments in which ‘dry’ axes were allowed to imbibe 3H-lebeled adenosine solution for 4 and 12 h in the presence of α-amanitin, it was found that poly A+RNA was newly synthesized to some extent in axes as early as 4 h after the onset of imbibition and that the drug effectively inhibited the poly A+RNA synthesis. The results may indicate the occurrence of stored mRNA in embryonic axes ofV. unguiculataseeds.