Suppressors of yeast actin mutations.
Suppressors of yeast actin mutations.
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DOI：
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发表时间：
1989-04
期刊：
影响因子：
3.3
通讯作者：
P. Novick;B. Osmond;D. Botstein
中科院分区：
文献类型：
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作者：
P. Novick;B. Osmond;D. Botstein
选择了同时获得冷敏感生长表型的酿酒酵母单个肌动蛋白基因的温度敏感突变(Act1-1)的抑制子。通过互补试验确定了5个基因，称为肌动蛋白抑制基因(SAC)；抑制和冷敏感表型都是隐性的。对其中3个基因(SAC1、SAC2和SAC3)进行了广泛的遗传和表型分析，包括分子克隆。对于肌动蛋白等位基因，抑制被发现是等位基因特异性的。SAC突变体，即使在ACT1+的遗传背景下，也表现出与肌动蛋白突变体相似的表型，特别是细胞内肌动蛋白的异常组织和细胞表面的甲壳素沉积。这些结果被解释为与SAC基因编码与肌动蛋白相互作用的蛋白质的想法一致，推测SAC基因可能是酵母肌动蛋白细胞骨架组装或稳定性的组成部分或控制器。人们注意到了SAC1基因的两个意想不到的特性。基因的破坏表明，只有在温度低于17摄氏度的情况下，它的功能才是必需的，当与Act1-2结合时，所有SAC1等位基因都是无效的。这些特性是在酵母肌动蛋白细胞骨架进化的背景下解释的。
Suppressors of a temperature-sensitive mutation (act1-1) in the single actin gene of Saccharomyces cerevisiae were selected that had simultaneously acquired a cold-sensitive growth phenotype. Five genes, called SAC (suppressor of actin) were defined by complementation tests; both suppression and cold-sensitive phenotypes were recessive. Three of the genes (SAC1, SAC2 and SAC3) were subjected to extensive genetic and phenotypic analysis, including molecular cloning. Suppression was found to be allele-specific with respect to actin alleles. The sac mutants, even in ACT1+ genetic backgrounds, displayed phenotypes similar to those of actin mutants, notably aberrant organization of intracellular actin and deposition of chitin at the cell surface. These results are interpreted as being consistent with the idea that the SAC genes encode proteins that interact with actin, presumably as components or controllers of the assembly or stability of the yeast actin cytoskeleton. Two unexpected properties of the SAC1 gene were noted. Disruptions of the gene indicated that its function is essential only at temperatures below about 17 degrees and all sac1 alleles are inviable when combined with act1-2. These properties are interpreted in the context of the evolution of the actin cytoskeleton of yeast.