Accumulation of 8-nitroguanine in human gastric epithelium induced by Helicobacter pylori infection
Accumulation of 8-nitroguanine in human gastric epithelium induced by Helicobacter pylori infection
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DOI：
10.1016/j.bbrc.2004.04.193
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发表时间：
2004-06-25
影响因子：
3.1
通讯作者：
Kawanishi, S
中科院分区：
文献类型：
--
作者：
Ma, N;Adachi, Y;Kawanishi, S
幽门螺杆菌感染引起慢性炎症，可导致胃癌。双免疫荧光标记研究表明，幽门螺杆菌感染的胃炎患者胃腺上皮中8-硝基鸟嘌呤和8-氧-7,8-二氢-2'-脱氧鸟嘌呤（8-oxodG）水平明显高于未感染的胃炎患者。与未感染幽门螺杆菌的患者相比，幽门螺杆菌感染患者的胃腺体上皮细胞中增殖细胞核抗原显著积累，增殖细胞核抗原是胃癌的预后因素，其积累与8-硝基鸟嘌呤和8-oxodG的形成密切相关。提示幽门螺杆菌感染对胃上皮细胞的亚硝化和氧化性DNA损伤及其增殖可能导致胃癌发生。8-硝基鸟嘌呤不仅可以作为一种有前景的炎症生物标志物，而且可以作为慢性幽门螺杆菌感染后胃癌发生风险的有用指标。(C) 2004爱思唯尔公司版权所有。
Helicobacter pylori infection causes chronic inflammation, which can lead to gastric carcinoma. A double immunofluorescence labeling study demonstrated that the level of 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) apparent in gastric gland epithelium was significantly higher in gastritis patients with H. pylori infection than in those without infection. A significant accumulation of proliferating cell nuclear antigen, a prognostic factor for gastric cancer, was observed in gastric gland epithelial cells in patients with H. pylori infection as compared to those without infection, and its accumulation was closely correlated with the formation of 8-nitroguanine and 8-oxodG. These results suggest that nitrosative and oxidative DNA damage in gastric epithelial cells and their proliferation by H. pylori infection may lead to gastric carcinoma. 8-Nitroguanine could be not only a promising biomarker for inflammation but also a useful indicator of the risk of gastric cancer development in response to chronic H. pylori infection. (C) 2004 Elsevier Inc. All rights reserved.