C-FOS ACTIVITY MAPPING REVEALS DIFFERENTIAL EFFECTS OF NORADRENALINE AND SEROTONIN DEPLETION ON THE REGULATION OF OCULAR DOMINANCE PLASTICITY IN RATS
C-FOS ACTIVITY MAPPING REVEALS DIFFERENTIAL EFFECTS OF NORADRENALINE AND SEROTONIN DEPLETION ON THE REGULATION OF OCULAR DOMINANCE PLASTICITY IN RATS
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DOI：
10.1016/j.neuroscience.2013.01.013
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发表时间：
2013-04-03
期刊：
影响因子：
3.3
通讯作者：
Watanabe, Y.
中科院分区：
文献类型：
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作者：
Nakadate, K.;Imamura, K.;Watanabe, Y.
中枢去甲肾上腺素能和肾上腺素能系统在眼优势可塑性活动依赖性调节中的作用一直是一个有争议的问题。使用c-Fos活性映射，我们已经开发出一种新的，直接的方法来衡量的强度眼优势可塑性：c-Fos免疫阳性细胞的数量在第四层的大鼠视觉皮层（OC 1B），同侧的刺激眼，是一个敏感和可靠的措施单眼剥夺的影响。应用这种新的方法，在这里，我们研究了独特的修改的程度c-Fos的表达诱导的视觉皮层，在内源性去甲肾上腺素（NA）和5-羟色胺（5-HT）在皮层中显着减少，分别由特定的药理学试剂。腹腔注射N-（2-氯乙基）-N-乙基-2-溴苄胺（DSP 4）和p-氯苯丙氨酸（pCPA）分别选择性损害NA和5 HT神经末梢和纤维。在视觉皮层与强烈减少NA，c-Fos免疫阳性细胞的数量被发现仍然显着减少，在响应于刺激的剥夺的眼睛，而通过开放的眼睛刺激的预期增加c-Fos免疫反应强烈抑制，显示值没有不同的单眼刺激在正常大鼠。与此相反，在视觉皮层与强烈减少5 HT没有预期的减少被发现在响应于刺激的剥夺的眼睛，而通常是正常动物的情况下，一个显着的增加仍然诱导在响应于睁眼刺激。这些研究结果表明，去甲肾上腺素能和肾上腺素能系统调节眼优势（OD）的可塑性不同：在NA耗尽皮层的预期增加c-Fos表达的睁眼刺激没有看到由于强烈的抑制，而在5 HT耗尽，预期减少c-Fos表达没有实现由于强烈的抑制。本研究结果与c-Fos活性映射方法表明一种新的可能性的差异调节OD可塑性的两种类型的共同单胺能系统。(C)2013年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The roles of the central noradrenergic and serotonergic system in the activity-dependent regulation of ocular dominance plasticity have been a contentious issue. Using c-Fos activity mapping, we have developed a new, straightforward method to measure the strength of ocular dominance plasticity: the number of c-Fos-immunopositive cells in layer IV of rat visual cortex (Oc1B), ipsilateral to the stimulated eye, is a sensitive and reliable measure of the effects of monocular deprivation. Applying this new method, here we studied the unique modification of the degree of c-Fos expression induced in the visual cortex, in that endogenous noradrenaline (NA) and serotonin (5HT) in the cortex were significantly reduced, respectively by specific pharmacological agents. Intraperitoneal injections of N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4) and p-chlorophenylalanine (pCPA) selectively impair NA-and 5HT-containing nerve terminals and fibers, respectively. In the visual cortex with strongly reduced NA, the number of c-Fos-immunopositive cells was found remaining significantly decreased in response to stimulation of the deprived eye, while by open eye stimulation the expected increase in c-Fos-immunoreactivity was strongly suppressed, showing values not different from those obtained by monocular stimulation in the normal rats. In contrast, in the visual cortex with strongly reduced 5HT no expected decrease was found in response to stimulation of the deprived eye, while, as is usually the case for the normal animals, a significant increase was still induced in response to open eye stimulation. These findings suggest that the noradrenergic and serotonergic system regulate ocular dominance (OD) plasticity differently: in the NA-depleted cortex the expected increase in c-Fos expression by open eye stimulation was not seen due to strong suppression, whereas in 5HT-depletion, the expected decrease in c-Fos expression was not materialized due to strong suppression. The present findings with c-Fos activity mapping method indicated a novel possibility of the differential regulation of OD plasticity by two types of common monoaminergic systems. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.