Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva.
Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva.
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DOI：
10.17912/micropub.biology.000863
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发表时间：
2023
影响因子：
--
通讯作者：
Sundaram, Meera V
中科院分区：
文献类型：
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作者：
Barker, Trevor J;Chan, Fung-Yi;Carvalho, Ana X;Sundaram, Meera V
梭Elegans外阴是极化的上皮管，其作为细胞-细胞信号传导、细胞命运特化和管发生的模型已被广泛研究。在这里，我们使用内源性融合显示血影蛋白细胞骨架在该器官中是极化的，传统的β-血影蛋白（β-70）仅在基底外侧膜发现，β-重血影蛋白（SMA-1）仅在顶膜发现。唯一的α-血影蛋白（SPC-1）存在于两个位置，但需要SMA-1的顶端定位。因此，β血影蛋白是外阴细胞膜和极性的极好标志物。
The C. elegans vulva is a polarized epithelial tube that has been studied extensively as a model for cell-cell signaling, cell fate specification, and tubulogenesis. Here we used endogenous fusions to show that the spectrin cytoskeleton is polarized in this organ, with conventional beta-spectrin ( UNC-70 ) found only at basolateral membranes and beta heavy spectrin ( SMA-1 ) found only at apical membranes. The sole alpha-spectrin ( SPC-1 ) is present at both locations but requires SMA-1 for its apical localization. Thus, beta spectrins are excellent markers for vulva cell membranes and polarity.