THE CENTROMERE SPECIFIC HISTONE CENP-A IS SELECTIVELY RETAINED IN DISCRETE FOCI IN MAMMALIAN SPERM NUCLEI
THE CENTROMERE SPECIFIC HISTONE CENP-A IS SELECTIVELY RETAINED IN DISCRETE FOCI IN MAMMALIAN SPERM NUCLEI
复制标题
DOI：
10.1007/bf00337600
复制
发表时间：
1990-12-01
期刊：
影响因子：
1.6
通讯作者：
MARGOLIS, RL
中科院分区：
文献类型：
--
作者：
PALMER, DK;ODAY, K;MARGOLIS, RL
命名为CENP-A的17 kDa人类自身抗原是一种着丝粒特异性组蛋白。我们在这里证明了CENP-A存在于牛起源的组织中，并在成熟精子中定量保留。这一结果是惊人的，因为哺乳动物精子发生的一个显著特征是用精蛋白取代大多数体细胞和睾丸特异的组蛋白。间接免疫荧光研究进一步表明，CENP-A保留在离散区域的精子核中，而不是分散在整个精子头上。这些观察表明，CENP-A是着丝粒的一个重要功能成分，并且先前存在的CENP-A：DNA相互作用可能在早期胚胎发育过程中组织父亲基因组的着丝粒方面起重要作用。
The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic and testes specific histones with protamines. Indirect immunofluorescence studies further show that CENP-A is retained in sperm nuclei in discrete foci, rather than being dispersed throughout the sperm head. These observations suggest that CENP-A is a functionally important component of centromeres, and that pre-existing CENP-A:DNA interactions are likely to be important in organizing the centromeres of the paternal genome during early embryogenesis.