A study on the effect of zinc oxide and zinc peroxide nanoparticles to enhance angiogenesis-pro-angiogenic grafts for tissue regeneration applications
A study on the effect of zinc oxide and zinc peroxide nanoparticles to enhance angiogenesis-pro-angiogenic grafts for tissue regeneration applications
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DOI：
10.1016/j.matdes.2017.07.023
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发表时间：
2017-10-15
影响因子：
8.4
通讯作者：
Rehman, Ihtesham Ur
中科院分区：
文献类型：
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作者：
Ahtzaz, Samreen;Nasir, Muhammad;Rehman, Ihtesham Ur
血管生成是一个由现有血管形成新的小血管的过程，对于正确和快速的组织愈合至关重要。由于活性氧（ROS）具有促进血管生成的能力，本研究合成了包封壳聚糖（CS）和纤维素基水凝胶的氧化锌（ZnO）和过氧化锌（ZnO2）纳米颗粒（NP’s）。与ZnO相比，ZnO2是相对较强的氧化剂，因此，在本研究中，我们假设ZnO2比ZnO具有更好的血管生成潜力。制备了三种类型的水凝胶；对照水凝胶（不含纳米颗粒）、含ZnO NP的水凝胶和含ZnO2 NP的水凝胶。采用扫描电子显微镜（SEM）、傅里叶红外光谱（FTIR）和x射线衍射（XRD）对水凝胶的结构进行了表征。在PBS、PBS/H2O2和PBS/溶菌酶溶液中考察其降解能力。用CAM实验研究了这些材料的血管生成行为，发现ZnO2基材料的血管生成能力显著提高。(C) 2017 Elsevier Ltd.版权所有。
Angiogenesis is a process of formation of new small blood vessels from existing vessels and is very critical for proper and rapid tissue healing. As reactive oxygen species (ROS) are known for their ability to promote angiogenesis, in this study zinc oxide (ZnO) and zinc peroxide (ZnO2) nano-particles (NP's) encapsulated chitosan (CS) and cellulose based hydrogels were synthesized. ZnO2 is comparatively stronger oxidizing agent as compared to ZnO, therefore, in this study it was hypothesized that ZnO2 would deliver better angiogenic potential than ZnO. Three types of hydrogels were prepared; control hydrogel (without nano-particles), hydrogel having ZnO NP's and hydrogel having ZnO2 NP's. The hydrogels were characterized by scanning electron microscopy (SEM) for structural morphology, Fourier transform infrared spectroscopy (FTIR) for chemical functional groups analyses and X-ray Diffraction (XRD) to investigate the crystalline or amorphous structure of NP's. The solution absorption capacity was tested in PBS and degradation was investigated in PBS, PBS/H2O2 and PBS/lysozyme solutions. The angiogenic behavior of these materials was studied in chorioallantoic membrane (CAM) assay and ZnO2 based material showed significant higher angiogenesis. (C) 2017 Elsevier Ltd. All rights reserved.