The structure of the gene coding for the phosphorylated ribosomal protein S10 in yeast.
The structure of the gene coding for the phosphorylated ribosomal protein S10 in yeast.
复制标题
酵母中磷酸化核糖体蛋白 S10 的编码基因结构。
DOI：
10.1093/nar/10.19.5869
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发表时间：
1982
影响因子：
14.9
通讯作者：
R. Planta
中科院分区：
文献类型：
--
作者：
R. Leer;M. M. van Raamsdonk;C. Molenaar;L. H. Cohen;W. Mager;R. Planta
从以前对克隆的酵母核糖体蛋白基因的研究中，我们获得了大量含有内含子的证据[Bollen等人。(1982)基因18，29-38]。在温度敏感的rna 2-突变转录中，这些基因导致前体RNA在限制性温度下积累。这些前体mRNAs比各自成熟的mRNAs长数百个核苷酸。其中一个核糖体蛋白基因的分裂特性，即。通过序列分析，进一步确定了主要的磷酸化小亚基蛋白S10的编码基因。该插入序列在第二密码子之后中断编码序列，其长度为352个核苷酸。基因组Southern杂交表明，该基因在酵母基因组中是复制的。根据序列分析推算出S10的相对分子质量为31462 da1。将酵母核糖体蛋白S10与大鼠肝脏核糖体蛋白S6的N末端氨基酸序列进行比较，发现两者具有显著的同源性。此外，在酵母核糖体蛋白的C末端存在Arg-Ala-Ser-Ser-Leu-Lys序列，该序列与大鼠肝脏蛋白S6的磷酸化位点非常相似。
From previous studies on cloned yeast ribosomal protein genes we obtained evidence that a large number of them contain an intron [Bollen et al. (1982) Gene 18, 29-38]. In the temperature-sensitive rna2-mutant transcription of these genes leads to the accumulation of precursor RNAs at the restrictive temperature. These precursor mRNAs are several hundreds of nucleotides longer than the respective mature mRNAs. The split character of one of these ribosomal protein genes, viz. the gene coding for the major phosphorylated small-subunit protein S10, was further established by sequence analysis. The intervening sequence interrupts the coding sequence after the second codon and has a length of 352 nucleotides. Genomic Southern hybridizations with a DNA fragment carrying part of the S10-gene revealed that this gene is duplicated on the yeast genome. The molecular weight of S10 as deduced from the sequence analysis was estimated to be 31462 dal. Comparison of the N-terminal aminoacid sequence of the yeast ribosomal protein S10 with that of ribosomal protein S6 from rat liver revealed a striking homology between both proteins. Moreover, at the C-terminal end of the yeast ribosomal protein the sequence Arg-Ala-Ser-Ser-Leu-Lys is present which is very similar to the phosphorylation site of the rat liver protein S6.