Glucuronoarabinoxylans from maize kernel cell walls are more complex than those from sorghum kernel cell walls
Glucuronoarabinoxylans from maize kernel cell walls are more complex than those from sorghum kernel cell walls
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DOI：
10.1016/s0144-8617(00)00154-5
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发表时间：
2000-11-01
影响因子：
11.2
通讯作者：
Voragen, AGJ
中科院分区：
文献类型：
--
作者：
Huisman, MMH;Schols, HA;Voragen, AGJ
从玉米籽粒中分离出水不可提取固体（WUS）。它们含有7%的蛋白质、8%的淀粉和57%的非淀粉多糖。这些NSP主要由葡萄糖、木糖、阿拉伯糖和葡萄糖醛酸组成。采用饱和Ba(OH)(2)溶液（BE1提取物）和蒸馏水（BE2提取物）顺序提取玉米WUS中的葡萄糖醛酸糖木聚糖。纤维素残留在不溶物中。提取物的糖苷键组成及其对内切木聚糖酶的抗性表明，提取的葡萄糖醛酸阿拉伯糖木聚糖被高度取代。在玉米BE1提取物中，25%的木糖未被取代，38%的木糖被单取代，15%的木糖被双取代。定义了一种新的替代度度量方法。玉米BE1阿拉伯木聚糖的替代度为87%，高于高粱BE1阿拉伯木聚糖的70%。玉米BE1中的葡萄糖醛酸阿拉伯糖木聚糖可以被一种来自葎草属植物的商业酶制剂Ultraflo的亚组分降解。该消化产物含有一系列低聚物：戊糖(n)、戊糖(n)GlcA、戊糖(n)己糖和戊糖(n)GlcA(2)。(C) 2000 Elsevier Science Ltd.版权所有。
Water-unextractable solids (WUS) were isolated from maize kernels. They contained 7% of protein, 8% of starch and 57% of non-starch polysaccharides (NSP). These NSP were composed mainly of glucose, xylose, arabinose, and glucuronic acid. Sequential extractions with a saturated Ba(OH)(2)-solution (BE1 extract), and distilled water (BE2 extract) were used to solubilise glucuronoarabinoxylans from maize WUS. Cellulose remained in the insoluble residue. The glycosidic linkage composition of the extracts and their resistance to endo-xylanase treatment indicated that the extracted glucuronoarabinoxylans were highly substituted. In the maize BE1 extract 25% of the xylose was unsubstituted, 38% was monosubstituted and 15% was disubstituted. A new measure for the degree of substitution is defined. The resulting degree of substitution for maize BE1 arabinoxylan (87%) is higher than for sorghum BE1 arabinoxylan (70%). The glucuronoarabinoxylans in maize BE1 can be degraded by a sub-fraction of Ultraflo, a commercial enzyme preparation from Humicola insolens. The digest contains a number of series of oligomers: pentose(n), pentose(n)GlcA, pentose(n)hexose, and pentose(n)GlcA(2). (C) 2000 Elsevier Science Ltd. All rights reserved.