Differential RNA expression profiles and competing endogenous RNA-associated regulatory networks during the progression of atherosclerosis
Differential RNA expression profiles and competing endogenous RNA-associated regulatory networks during the progression of atherosclerosis
复制标题
动脉粥样硬化进展过程中的差异RNA表达谱和竞争性内源RNA相关调控网络
DOI：
10.2217/epi-2020-0252
复制
发表时间：
2021-01-01
期刊：
影响因子：
3.8
通讯作者：
Tian, Jiangtian
中科院分区：
文献类型：
--
作者：
Fu, Yahong;Sun, Changbin;Tian, Jiangtian
目的：目的：研究动脉粥样硬化（AS）发生发展过程中mRNA和ncRNA的差异表达谱及竞争性内源性RNA相关调控网络。材料和方法：我们系统地分析了来自AS不同阶段的样本的全转录组测序，以评估其长非编码RNA（lncRNA）、环状RNA（circRNA）、miRNA和mRNA谱。结果：构建了3个AS相关的竞争性内源RNA调控网络，包括差异表达的circRNAs、lncRNAs、miRNAs和mRNAs。Gene Ontology和京都基因和基因组百科全书分析显示，网络中的circRNA富集在脂质代谢过程中，并参与了PPAR信号通路。此外，lncRNA与受体活性、肌原纤维和心血管系统发育有关。结论：本研究结果进一步阐明了AS不同阶段的调控机制，为AS的治疗提供了新的思路和靶点。
Aim: To identify differential mRNA and ncRNA expression profiles and competing endogenous RNA-associated regulatory networks during the progression of atherosclerosis (AS). Materials & methods: We systematically analyzed whole-transcriptome sequencing of samples from different stages of AS to evaluate their long noncoding RNA (lncRNA), circular RNA (circRNA), miRNA and mRNA profiles. Results: We constructed three AS-related competing endogenous RNA regulatory networks of differentially expressed circRNAs, lncRNAs, miRNAs and mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed that the circRNAs in the network were enriched in lipid metabolic processes and participated in the PPAR signaling pathway. Furthermore, lncRNAs were related to receptor activity, myofibrils and cardiovascular system development. Conclusion: The current findings further clarified the regulatory mechanisms at different stages of AS and may provide new ideas and targets for AS.