Color-discriminating retinal configurations of sensory rhodopsin I by photo-irradiation solid-state NMR spectroscopy.
Color-discriminating retinal configurations of sensory rhodopsin I by photo-irradiation solid-state NMR spectroscopy.
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DOI：
10.1002/anie.201309258
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发表时间：
2014-07
影响因子：
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通讯作者：
H. Yomoda;Yoshiteru Makino;Yuya Tomonaga;T. Hidaka;I. Kawamura;Takashi Okitsu;A. Wada;Y. Sudo;A. Naito
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文献类型：
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作者：
H. Yomoda;Yoshiteru Makino;Yuya Tomonaga;T. Hidaka;I. Kawamura;Takashi Okitsu;A. Wada;Y. Sudo;A. Naito
SRI（感觉视紫红质 I）可以区分多种颜色以实现引诱和驱避趋光性。旨在揭示颜色依赖性机制的研究表明，SRI 不仅在光生物学中而且在光化学中都是一个具有挑战性的系统。在 SRI 的光反应过程中，M-中间体（引诱剂）通过吸收蓝光转变为 P-中间体（驱避剂）。因此，SRI 然后循环回到 G 状态。使用原位光照射固态核磁共振光谱法，用[20-(13)C]retnal-SrSRI 的(13)C NMR 信号监测光反应。 M-中间体在 -40 °C 下通过 520 nm 照射被捕获。随后在 365 nm 处照射，将其转化为 P-中间体。这些结果表明，在 365 nm 的照射下，G 态可以直接转变为 P 中间体。因此，固定捕获的 M- 和 P- 中间体分别负责正趋光性和负趋光性。
SRI (sensory rhodopsin I) can discriminate multiple colors for the attractant and repellent phototaxis. Studies aimed at revealing the color-dependent mechanism show that SRI is a challenging system not only in photobiology but also in photochemistry. During the photoreaction of SRI, an M-intermediate (attractant) transforms into a P-intermediate (repellent) by absorbing blue light. Consequently, SRI then cycles back to the G-state. The photoreactions were monitored with the (13)C NMR signals of [20-(13)C]retnal-SrSRI using in situ photo-irradiation solid-state NMR spectroscopy. The M-intermediate was trapped at -40 °C by illumination at 520 nm. It was transformed into the P-intermediate by subsequent illumination at 365 nm. These results reveal that the G-state could be directly transformed to the P-intermediate by illumination at 365 nm. Thus, the stationary trapped M- and P-intermediates are responsible for positive and negative phototaxis, respectively.