In vivo cerebrovascular measurement combining diffuse near-infrared absorption and correlation spectroscopies
In vivo cerebrovascular measurement combining diffuse near-infrared absorption and correlation spectroscopies
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DOI：
10.1088/0031-9155/46/8/302
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发表时间：
2001-08-01
影响因子：
3.5
通讯作者：
Yodh, AG
中科院分区：
文献类型：
--
作者：
Cheung, C;Culver, JP;Yodh, AG
我们结合联合收割机两种近红外扩散光学技术来研究功能性大鼠脑中血流量、血红蛋白浓度和血氧饱和度的变化。弥散相关光谱（或流量计）监测脑血流的变化，而不使用示踪剂清除的原则，通过测量移动血细胞引起的光学相移。近红外吸收光谱同时测量两个波长的组织吸收，以确定同一组织体积中的血红蛋白浓度和血氧饱和度。该光学探头是非侵入性的，并且通过完整的颅骨使用。该技术的实用性证明在体内通过测量在高碳酸血症大鼠脑的区域血管动力学的时间变化。在高碳酸血症的脑血流量，血红蛋白浓度和血氧饱和度的时间和空间变化进行了比较与其他测量在文献中，和一个定量分析表明我们的血管反应的联合观察的自洽性。
We combine two near-infrared diffuse optical techniques to study variations of blood flow, haemoglobin concentration, and blood oxygen saturation in the functioning rat brain. Diffuse correlation spectroscopy (or flowmetry) monitors changes in the cerebral blood flow, without the use of the principles of tracer clearance, by measuring the optical phase-shifts caused by moving blood cells. Near-infrared absorption spectroscopy concurrently measures tissue absorption at two wavelengths to determine haemoglobin concentration and blood oxygen saturation in this same tissue volume. This optical probe is non-invasive and was employed through the intact skull. The utility of the technique is demonstrated in vivo by measuring the temporal changes in the regional vascular dynamics of rat brain during hypercapnia. Temporal and spatial variations of cerebral blood flow, haemoglobin concentration and blood oxygen saturation during hypercapnia are compared with other measurements in the literature, and a quantitative analysis demonstrating the self-consistency of our combined observations of vascular response is presented.