Influence of development and joint pathology on stromelysin enzyme activity in equine synovial fluid
Influence of development and joint pathology on stromelysin enzyme activity in equine synovial fluid
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DOI：
10.1136/ard.59.2.155
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发表时间：
2000-02-01
影响因子：
27.4
通讯作者：
van Weeren, PR
中科院分区：
文献类型：
--
作者：
Brama, PAJ;TeKoppele, JM;van Weeren, PR
研究基质溶素（MMP-3）活性在不同发育阶段和常见关节疾病中的作用，在马的SF中，用荧光酶活性测定法测定了基质溶素活性在胎儿、青少年和成年马的正常关节的SF中，以及在患有发育性骨科疾病骨软骨病（OC）或骨关节炎（OA）的马的SF中。此外，MMP-3活性表示为先前报道的相同SF样品中的一般MMP活性的比率。
Objective-To investigate the role of stromelysin (MMP-3) activity in synovial fluid (SF) at different stages of development and in common joint disorders in the horse.Methods-Stromelysin activity was determined with a fluorogenic enzyme activity assay in SF of normal joints of fetal, juvenile and adult horses, and in SF of horses suffering from the developmental orthopaedic disease osteochondrosis (OC) or osteoarthritis (OA). Additionally, MMP-3 activity was expressed as a ratio of previously reported general MMP activity in the same SF samples.Results-The levels of active stromelysin were 30-fold to 80-fold higher in SF from fetal horses than in SF from juvenile and mature animals (p