The transcriptional regulator NtrC controls glucose-6-phosphate dehydrogenase expression and polyhydroxybutyrate synthesis through NADPH availability in Herbaspirillum seropedicae.
The transcriptional regulator NtrC controls glucose-6-phosphate dehydrogenase expression and polyhydroxybutyrate synthesis through NADPH availability in Herbaspirillum seropedicae.
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DOI：
10.1038/s41598-017-12649-0
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发表时间：
2017-10-19
影响因子：
4.6
通讯作者：
Müller-Santos M
中科院分区：
文献类型：
--
作者：
Sacomboio ENM;Kim EYS;Ruchaud Correa HL;Bonato P;de Oliveira Pedrosa F;de Souza EM;Chubatsu LS;Müller-Santos M
NTR系统是细菌氮代谢的主要调节因子。尽管它在含氮分子的同化、生物合成和循环中的作用广为人知，但人们对它在碳代谢中的作用知之甚少。在这项工作中，我们提出了NTR系统在控制NADPH浓度和依赖还原辅酶的分子生物合成方面的一个新方面。我们证明了NTRC突变菌株积累了高水平的聚羟基丁酸酯(PHB)，达到了比亲本菌株高2倍的水平。在没有NTRC的情况下，葡萄糖-6-磷酸脱氢酶(由zwf编码)的活性增加了2.8倍，从而导致NADPH/NADP+比率增加了2.1倍。GFP融合结果表明，zwf的表达同样受NTRC的调控。NADPH利用率的增加刺激了聚羟基丁酸酯的产生，而不考虑介质中的C/N比。突变体NTRC对H_2O_2暴露的抗性更强，并在面临氧化条件时控制了ROS的生长，这是与PHB含量增加相关的表型。
The NTR system is the major regulator of nitrogen metabolism in Bacteria. Despite its broad and well-known role in the assimilation, biosynthesis and recycling of nitrogenous molecules, little is known about its role in carbon metabolism. In this work, we present a new facet of the NTR system in the control of NADPH concentration and the biosynthesis of molecules dependent on reduced coenzyme in Herbaspirillum seropedicae SmR1. We demonstrated that a ntrC mutant strain accumulated high levels of polyhydroxybutyrate (PHB), reaching levels up to 2-fold higher than the parental strain. In the absence of NtrC, the activity of glucose-6-phosphate dehydrogenase (encoded by zwf) increased by 2.8-fold, consequently leading to a 2.1-fold increase in the NADPH/NADP+ ratio. A GFP fusion showed that expression of zwf is likewise controlled by NtrC. The increase in NADPH availability stimulated the production of polyhydroxybutyrate regardless the C/N ratio in the medium. The mutant ntrC was more resistant to H2O2 exposure and controlled the propagation of ROS when facing the oxidative condition, a phenotype associated with the increase in PHB content.
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影响因子：
4.2
作者：
Kadowaki MA;Müller-Santos M;Rego FG;Souza EM;Yates MG;Monteiro RA;Pedrosa FO;Chubatsu LS;Steffens MB
通讯作者：
Steffens MB
影响因子：
2.1
作者：
HAYWOOD, GW;ANDERSON, AJ;DAWES, EA
通讯作者：
DAWES, EA
影响因子：
5.2
作者：
Alves LP;Teixeira CS;Tirapelle EF;Donatti L;Tadra-Sfeir MZ;Steffens MB;de Souza EM;de Oliveira Pedrosa F;Chubatsu LS;Müller-Santos M
通讯作者：
Müller-Santos M
影响因子：
3.7
作者：
Kocharin K;Chen Y;Siewers V;Nielsen J
通讯作者：
Nielsen J
影响因子：
4.9
作者：
Chubatsu, Leda Satie;Monteiro, Rose Adele;Pedrosa, Fabio de Oliveira
通讯作者：
Pedrosa, Fabio de Oliveira