Monoclonal anti-acetylcholine receptor antibodies can cause experimental myasthenia.
Monoclonal anti-acetylcholine receptor antibodies can cause experimental myasthenia.
复制标题
单克隆抗乙酰胆碱受体抗体可引起实验性肌无力。
作者：
Richman,DP;Gomez,CM;Berman,PW;Burres,SA;Fitch,FW;Arnason,BG
重症肌无力 (MG) 是一种因针对肌神经连接处烟碱乙酰胆碱受体 (AChR) 的自身免疫反应而发生的疾病1-3。针对复杂抗原（例如AChR）产生的抗血清包含与抗原分子上的各种决定簇反应的抗体混合物。针对任何单一决定簇的抗体可以属于多种免疫球蛋白类别和独特型。由克隆淋巴细胞-骨髓瘤杂交瘤细胞系产生的抗体提供了一种分析构成多克隆抗血清的不同成分的方法4，并评估每种成分对体内整体免疫反应的相对贡献5,6。我们已将该技术应用于 MG 自身免疫反应的研究。我们在这里证明，某些单克隆抗鱼雷AChR抗体，当静脉注射到正常大鼠体内时，会诱发急性肌无力综合征。因此，与单一抗原决定簇反应的单一分子种类的抗体的结合可以导致自身免疫性疾病的所有表现。
Myasthenie gravis (MG) is a disease which occurs as a consequence of an autoimmune response directed against the nicotinic acetylcholine receptor (AChR) of the myoneural junction1–3. Antisera raised against complex antigens such as AChR comprise a mixture of antibodies reactive with various determinants on the antigen molecule. The antibodies against any single determinant may be of several immunoglobulin classes and idiotypes. Antibodies produced by cloned lymphocyte–myeloma hybridoma cell lines have provided a way of analysing the diverse components making up a polyclonal antiserum4and assessing the relative contribution made by each to the overall immune reactionin vivo5,6. We have applied this technique to the investigation of the autoimmune response in MG. We demonstrate here that certain monoclonal anti-TorpedoAChR antibodies, when injected intravenously into normal rats, induce an acute myasthenie syndrome. Thus binding of a single molecular species of antibody reactive with a single antigenic determinant can result in all of the manifestations of an autoimmune disease.