FATTY-ACID OXIDATION BY ISOLATED PERFUSED WORKING HEARTS OF AGED RATS
FATTY-ACID OXIDATION BY ISOLATED PERFUSED WORKING HEARTS OF AGED RATS
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DOI：
10.1152/ajpendo.1977.232.3.e258
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发表时间：
1977-01-01
影响因子：
--
通讯作者：
SANADI, DR
中科院分区：
文献类型：
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作者：
ABUERREISH, GM;NEELY, JR;SANADI, DR
据报道，从老年大鼠心脏中分离的线粒体比年轻大鼠的线粒体具有更低的呼吸活性。为了确定这些变化的生理相关性，在灌注工作心脏制备中比较了年轻和老年大鼠心脏的代谢。在不同的心脏负荷下测量[14C]棕榈酸盐氧化为14CO2、耗氧量和核苷酸水平。来自老年动物的心脏进行较少的心脏工作，并且与组织质量成比例地利用较少的氧气和棕榈酸盐，但是消耗的氧气与产生的压力的比率没有改变。有一个小的，但显着的心脏效率下降，表示为耗氧率和心室压力发展之间的比率。总肉碱和长链酰基肉碱衍生物的组织水平大大降低，在老年人的心脏没有显着变化，游离CoA，乙酰CoA，或长链酰基CoA。腺嘌呤核苷酸水平在两组中无显著差异。结果似乎与分离的线粒体的体外研究一致。
It has been reported that mitochondria isolated from hearts of old rats have lower respiratory activity than mitochondria from young rats. In order to determine the physiological correlates of these changes, the metabolism of hearts from young and old rats has been compared in a perfused working heart preparation. The oxidation of [14C]palmitate to 14CO2, oxygen consumption, and nucleotide levels were measured under different cardiac workloads. The hearts from old animals performed less cardiac work and utilized less oxygen and palmitate in proportion to tissue mass, but the ratio of oxygen consumed to pressure developed was unaltered. There was a small but significant decrease in cardiac efficiency expressed as the ratio between the rate of oxygen consumed and ventricular pressure development. Tissue levels of total carnitine and long-chain acylcarnitine derivatives were greatly reduced in the older heart without significant change in free CoA, acetyl-CoA, or long-chain acyl-CoA. The adenine nucleotide levels were not significantly different in the two groups. The results appear consistent with the in vitro studies on isolated mitochondria.