On the metabolism of GM3 ganglioside in cultured human foreskin fibroblasts.
On the metabolism of GM3 ganglioside in cultured human foreskin fibroblasts.
复制标题
GM3神经节苷脂在培养的人包皮成纤维细胞中的代谢。
DOI：
10.1016/0006-291x(89)90799-7
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Sweeley,CC
中科院分区：
文献类型：
--
作者：
Swamy,MJ;Sweeley,CC
通过用[1- 3 H]-半乳糖标记培养的细胞48小时，然后进行48小时追踪，研究GM 3神经节苷脂在培养的人包皮成纤维细胞中的代谢。在碳水化合物链的己糖部分中发现了超过80%的与GM 3相关的放射性，而在唾液酸部分中观察到约12%的放射性。的己糖和唾液酸残基失去了42%和53%的初始放射性，分别在追逐期间，表明这些糖残基的GM 3在生长培养物中的活性代谢。
The metabolism of GM3 ganglioside in cultured human foreskin fibroblasts was investigated by labeling cultured cells with [1-3H]-galactose for 48 hours, followed by a 48 hour chase. More than 80% of the radioactivity associated with GM3 was found in the hexose portion of the carbohydrate chain, whereas approximately 12% of the radioactivity was observed in the sialic acid moiety. The hexose and sialic acid residues lost 42% and 53% of their initial radioactivity, respectively, during the chase period, indicating an active metabolism of these sugar residues of GM3in growing cultures.