Natural variation in rosette size under salt stress conditions corresponds to developmental differences between Arabidopsis accessions and allelic variation in the LRR-KISS gene.
Natural variation in rosette size under salt stress conditions corresponds to developmental differences between Arabidopsis accessions and allelic variation in the LRR-KISS gene.
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DOI：
10.1093/jxb/erw015
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发表时间：
2016-04
影响因子：
6.9
通讯作者：
Testerink C
中科院分区：
文献类型：
--
作者：
Julkowska MM;Klei K;Fokkens L;Haring MA;Schranz ME;Testerink C
对拟南芥自然变异的研究表明，耐盐性与植物发育和新候选基因的表达有关。拟南芥材料之间的自然变异是鉴定植物发育和逆境耐性机制的重要遗传资源。为了评估盐胁迫耐性的自然变异，在两个群体上进行了两个大规模的实验，每个群体包括160个拟南芥材料。收集了包括莲座丛投影面积、鲜重和干重在内的多个性状作为耐盐性的估计。我们的结果揭示了盐胁迫条件下莲座体大小与对照材料之间的发育差异之间的相关性，这表明总体上较大的植株更耐盐。当植物生长在严重的盐胁迫条件下时，这种相关性不那么明显。随后的全基因组关联研究揭示了与耐盐性相关的新候选基因，如LRR-KISS(At4g08850)、开花基因KH结构域蛋白和DUF1639蛋白。在盐胁迫条件下，高表达LRR-KISS的材料形成了更大的玫瑰花结。本研究确定的候选基因的等位基因变异的进一步鉴定将为更深入地了解由于促进地上部生长而产生的耐盐性的机制。
Study of natural variation of Arabidopsis reveals salinity tolerance to be linked to plant development and expression of novel candidate genes. Natural variation among Arabidopsis accessions is an important genetic resource to identify mechanisms underlying plant development and stress tolerance. To evaluate the natural variation in salinity stress tolerance, two large-scale experiments were performed on two populations consisting of 160 Arabidopsis accessions each. Multiple traits, including projected rosette area, and fresh and dry weight were collected as an estimate for salinity tolerance. Our results reveal a correlation between rosette size under salt stress conditions and developmental differences between the accessions grown in control conditions, suggesting that in general larger plants were more salt tolerant. This correlation was less pronounced when plants were grown under severe salt stress conditions. Subsequent genome wide association study (GWAS) revealed associations with novel candidate genes for salinity tolerance such as LRR-KISS (At4g08850), flowering locus KH-domain containing protein and a DUF1639-containing protein. Accessions with high LRR-KISS expression developed larger rosettes under salt stress conditions. Further characterization of allelic variation in candidate genes identified in this study will provide more insight into mechanisms of salt stress tolerance due to enhanced shoot growth.