Immunohistochemical diagnosis of Toxoplasma gondii: potential for cross-reactivity with Neospora caninum.
Immunohistochemical diagnosis of Toxoplasma gondii: potential for cross-reactivity with Neospora caninum.
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弓形虫的免疫组织化学诊断：与犬新孢子虫潜在的交叉反应。
DOI：
10.2307/3284407
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
D. Lindsay
中科院分区：
文献类型：
--
作者：
C. Sundermann;B. H. Estridge;M. S. Branton;C. R. Bridgman;D. Lindsay
将单克隆抗体（MAB 3F 5）与商业多克隆抗体在石蜡包埋组织切片中对弓形虫的特异性和与犬新孢子虫的交叉反应性进行比较。两种抗体制剂均可靠地识别T.弓形虫速殖子（RH分离株）在动物组织中，但并不总是标记组织囊肿（ME-49分离株）。商品化抗体与N.犬速殖子，但MAB 3F 5在采用免疫过氧化物酶、免疫荧光或免疫金方法时没有，也不与其他顶复体交叉反应，例如，猪等孢子虫，牛艾美耳球虫。克氏住肉孢子虫、哈蒙迪沙门氏菌或双遗传核孢子虫。免疫电镜显示MAB 3F 5与T.弓形虫速殖子在T.单克隆抗体3F 5可标记弓形虫速殖子，在38 kDa处有一条主带，在32 kDa处有一条次带，但对弓形虫速殖子的蛋白质无标记。犬速殖子或未感染的宿主细胞发生在超微结构水平或免疫印迹。
A monoclonal antibody (MAB 3F5) was compared with a commercial polyclonal antibody for specificity for Toxoplasma gondii and cross-reactivity with Neospora caninum in paraffin-embedded tissue sections. Both antibody preparations reliably recognized T. gondii tachyzoites (RH isolate) in animal tissues but did not always label tissue cysts (ME-49 isolate). The commercial antibody strongly cross-reacted with N. caninum tachyzoites, but MAB 3F5 did not when the immunoperoxidase, immunofluorescence, or immunogold procedures were employed, nor did it cross-react with other apicomplexans, e.g., Isospora suis, Eimeria bovis. Sarcocystis cruzi, Hammondia hammondi, or Caryospora bigenetica. Immumoelectron microscopy revealed that MAB 3F5 bound to dense granules in extracellular T. gondii tachyzoites. In western blots of T. gondii tachyzoites, a major band at 38 kDa and a minor band at 32 kDa were labeled by MAB 3F5, but no labeling of the proteins of N. caninum tachyzoites or uninfected host cells occurred at the ultrastructural level or in immunoblots.