IS CYTOSOLIC IONIZED CALCIUM REGULATING NEUTROPHIL ACTIVATION
IS CYTOSOLIC IONIZED CALCIUM REGULATING NEUTROPHIL ACTIVATION
复制标题
DOI：
10.1126/science.6310757
复制
发表时间：
1983-01-01
期刊：
影响因子：
56.9
通讯作者：
TSIEN, RY
中科院分区：
文献类型：
--
作者：
POZZAN, T;LEW, DP;TSIEN, RY
利用捕获在细胞质中的荧光指示剂，在完整的[人]中性粒细胞中测量了胞质电离Ca [Ca2+]i的浓度。与绕过细胞表面受体的特定钙离子载体离子霉素产生的相同或更大的[Ca2+]i相比，由趋化肽甲酰基-蛋氨酸-酰基-酰基-苯丙氨酸（FMLP）引起的[Ca2+]i的增加与超氧化物的产生和髓过氧化物酶的分泌程度要大得多。FMLP除了增加[Ca2+]i外，似乎还会产生一些重要的兴奋性信号，中性粒细胞的胞分泌和超氧化物的产生可能并不像人们普遍认为的那样仅仅依赖于[Ca2+]i。
The concentration of cytosolic ionized Ca [Ca2+]i, was measured in intact [human] neutrophils by use of a fluorescent indicator trapped in the cytoplasm. A given rise of [Ca2+]i elicited by the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (FMLP) was associated with a much greater degree of superoxide generation and myeloperoxidase secretion than was the same or larger [Ca2+]i produced by a specific Ca ionophore, ionomycin, which bypasses cell surface receptors. FMLP appears to generate some important excitatory signal in addition to a rise in [Ca2+]i, and exocytosis and superoxide generation in neutrophils may not be simply dependent on [Ca2+]i as is widely supposed.