Hyaluronan in articular cartilage: Analysis of hip osteoarthritis and osteonecrosis of femoral head
Hyaluronan in articular cartilage: Analysis of hip osteoarthritis and osteonecrosis of femoral head
复制标题
DOI：
10.1002/jor.25364
复制
发表时间：
2022-05
期刊：
影响因子：
--
通讯作者：
Jiarui Zhang;Y. Nishida;H. Koike;Kan Ito;Lisheng Zhuo;Kazuki Nishida;K. Kimata;Kunihiro Ikuta
中科院分区：
文献类型：
--
作者：
Jiarui Zhang;Y. Nishida;H. Koike;Kan Ito;Lisheng Zhuo;Kazuki Nishida;K. Kimata;Kunihiro Ikuta
透明质酸（HA）在维持高质量的软骨细胞外基质中起着至关重要的作用。已有研究报道了骨关节炎（OA）患者滑液中HA的变化，但对OA或特发性股骨头坏死（ONFH）患者关节软骨中HA的变化报道较少。最近报道KIAA1199具有强透明质酸酶活性。本研究的目的是阐明OA和ONFH中的HA代谢，特别是KIAA1199的参与。对人OA（n = 10）、ONFH（n = 10）和对照软骨（n = 7）进行KIAA1199和HA沉积的免疫组织化学分析。用竞争性HA ELISA和层析法分别测定HA的浓度和分子量。关于HA代谢相关分子，通过逆转录酶聚合酶链反应评估了HAS 1、HAS 2、HAS 3、HYAL 1、HYAL 2和KIAA 1199基因表达。组织学分析显示OA软骨中KIAA1199的过度表达，与ONFH和对照相比，其伴随着透明质酸结合蛋白（HABP）染色的减少。KIAA1199在ONFH塌陷区的软骨中几乎没有表达，伴随着HABP染色的轻微减少。OA软骨中HAS 2和KIAA1199的mRNA表达上调，而ONFH软骨中HA catalysts相关基因的mRNA表达大多呈下降趋势。OA软骨HA分子量增加，ONFH软骨HA分子量减少。ONFH中的HA代谢通常是惰性的，并且在OA中被激活，包括KIAA1199的高表达。有趣的是，OA软骨中HA的MW没有降低。
Hyaluronan (HA) plays crucial roles in the maintenance of high‐quality cartilage extracellular matrix. Several studies have reported the HA in synovial fluid in patients with osteoarthritis (OA), but few have described the changes of HA in articular cartilage of OA or idiopathic osteonecrosis of the femoral head (ONFH). KIAA1199 was recently reported to have strong hyaluronidase activity. The aim of this study was to clarify the HA metabolism in OA and ONFH, particularly the involvement of KIAA1199. Immunohistochemical analysis of KIAA1199 and HA deposition was performed for human OA (n = 10), ONFH (n = 10), and control cartilage (n = 7). The concentration and molecular weight (MW) of HA were determined by competitive HA ELISA and Chromatography, respectively. Regarding HA metabolism‐related molecules, HAS1, HAS2, HAS3, HYAL1, HYAL2, and KIAA1199 gene expression was assessed by reverse transcriptase polymerase chain reaction. Histological analysis showed the overexpression of KIAA1199 in OA cartilage, which was accompanied by decreased hyaluronic acid binding protein (HABP) staining compared with ONFH and control. Little KIAA1199 expression was observed in cartilage at the collapsed area of ONFH, which was accompanied by a slight decrease in HABP staining. The messenger RNA (mRNA) expression of HAS2 and KIAA1199 was upregulated in OA cartilage, while the mRNA expression of genes related to HA catabolism in ONFH cartilage showed mostly a downward trend. The MW of HA in OA cartilage increased while that in ONFH cartilage decreased. HA metabolism in ONFH is suggested to be generally indolent, and is activated in OA including high expression of KIAA1199. Interestingly, MW of HA in OA cartilage was not reduced.