Frameshift suppression Saccharomyces cerevisiae. II. Genetic properties of group II suppressors.
Frameshift suppression Saccharomyces cerevisiae. II. Genetic properties of group II suppressors.
复制标题
移码抑制酿酒酵母。
DOI：
10.1093/genetics/95.4.833
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发表时间：
1980
期刊：
影响因子：
3.3
通讯作者：
Fink,GR
中科院分区：
文献类型：
--
作者：
Culbertson,MR;Underbrink,KM;Fink,GR
已在酿酒酵母中鉴定出表现出与细菌移码抑制子类似行为的 ICR 诱导突变抑制子。酵母抑制剂分为两组。其中一组（第二组：SUFI、SUF3、SUF4、SUF5 和 SUF6）似乎包括一组信息抑制因子，其中抑制载体是甘氨酰-tRNA。本通讯中描述了 II 组抑制子的一些遗传特性。II 组移码突变的回复突变体 this4-519 和 leu2-3 已被表征，以确定由移码诱变剂 ICR-170 诱导的回复事件谱。分析了 73 个 ICR 诱导的共突变体。除一个携带SUF1等位基因的共变体外，所有携带SUF3或SUF5、SUF1、SUF3、SUF4和SUF6等位基因的共变体均在自发和紫外线诱导的共变体中出现。在这些实验过程中，其中一个抑制子被定位。SUF5（tRNAGLY1 的可能结构基因）位于 XV 染色体上的ade2 和ade9 之间。SUF1、SUF4 和SUF6 具有新颖的特性，并包含一个独特的抑制子子集。尽管这些抑制子彼此之间没有遗传联系，但它们具有几个共同特征，包括单倍体成对组合的致死性、tRNAGLY3isoacceptor活性降低以及携带细胞质遗传[PSI]元件的菌株中抑制效率提高。此外，携带SUFI、SUF4或SUF6的菌株表型不稳定，并以高频率产生有丝分裂Suf+分离子。这些分离子总是包含一个连锁的第二位点突变，该突变映射到抑制基因本身或邻近抑制基因本身。携带任何这些抑制因子的菌株也会产生有丝分裂分离子，表现出增强的抑制效率。导致该表型图的突变位于两个基因座，upf1和upf2。这些基因与任何第二组抑制子都没有遗传联系。为了检查大量变体，已经设计了允许积极选择抑制效率降低或增强的突变体的方法。这些相互作用的突变体在分子水平研究抑制功能方面的潜在用途强调了它们的重要性。
Suppressors of ICR-induced mutations that exhibit behavior similar to bacterial frameshift suppressors have been identified in the yeastSaccharomyces cerevisiae. The yeast suppressors have been divided into two groups. One of these groups (Group II:SUFI, SUF3, SUF4, SUF5andSUF6) appears to include a set of informational suppressors in which the vehicle of suppression is glycyl-tRNA. Some of the genetic properties of Group II suppressors are described in this communication.—Corevertants of the Group II frameshift mutationshis4-519andleu2-3have been characterized to determine the spectrum of reversion events induced by the frameshift mutagen ICR-170. Seventythree ICR-induced corevertants were analyzed. With the exception of one corevertant, which carried an allele ofSUF1,all carried alleles ofSUF3orSUF5, SUF1, SUF3, SUF4andSUF6were represented among spontaneous and UV-induced corevertants. In the course of these experiments one of the suppressors was mapped.SUF5,the probable structural gene for tRNAGLY1, is located betweenade2andade9on chromosomeXV.—SUF1, SUF4andSUF6have novel properties and comprise a distinct subset of suppressors. Although these suppressors show no genetic linkage to each other, they share several common features including lethality in haploid pairwise combinations, reduced tRNAGLY3isoacceptor activity and increased efficiency of suppression in strains carrying the cytoplasmically inherited [PSI] element. In addition, strains carryingSUFI, SUF4orSUF6are phenotypically unstable and give rise to mitotic Suf+segregants at high frequency. These segregants invariably contain a linked, second-site mutation that maps in or adjacent to the suppressor gene itself. Strains carrying any of these suppressors also give rise to mitotic segregants that exhibit enhanced efficiency of suppression; mutations responsible for this phenotype map at two loci,upf1andupf2. These genes show no genetic linkage to any of the Group II suppressors.—Methods that permit positive selection for mutants with decreased or enhanced efficiency of suppression have been devised in order to examine large numbers of variants. The importance of these interacting mutants is underscored by their potential utility in studying suppressor function at the molecular level.