EFFECT OF TRNA CONCENTRATION ON RATE OF PROTEIN SYNTHESIS
EFFECT OF TRNA CONCENTRATION ON RATE OF PROTEIN SYNTHESIS
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DOI：
10.1073/pnas.62.2.566
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发表时间：
1969-01-01
影响因子：
11.1
通讯作者：
ANDERSON, WF
中科院分区：
文献类型：
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作者：
ANDERSON, WF
两个来源弗罗姆.大肠杆菌的研究已经证明了tRNA种类的浓度可以调节信使RNA的翻译速率。(a)在1.5 × 10- 8 M至3.0 × 10- 6 M的浓度范围内，多聚-U-定向的C14-苯丙氨酸掺入蛋白质的速率受到刺激，后者代表tRNAPhe/70 S核糖体比率为7。(b)本文报道了弗罗姆.大肠杆菌中存在的识别提取物中存在的码字阿加和AGG的tRNA Arg的量限制。多肽合成的刺激与加入反应混合物中的tRNAArg物质的量成正比。在翻译水平上调节蛋白质合成速率的机制可能是由于限速tRNA种类的存在而减慢某些密码子处的多肽链增殖。
Twoin vitroprotein-synthesizing systems derived fromE. colihave been utilized to demonstrate that the concentration of a tRNA species can regulate the rate of translation of a messenger RNA. (a) The rate of poly-U-directed C14-phenylalanine incorporation into protein is stimulated by concentrations of tRNAPhefrom 1.5 × 10-8M to 3.0 × 10-6M, the latter representing a tRNAPhe/70Sribosome ratio of 7. (b) The rate of translation of poly A,G in a S-30 protein-synthesizing system derived fromE. coliis limited by the amount of tRNAArgrecognizing the codewords AGA and AGG present in the extract. Polypeptide synthesis can be stimulated in direct proportion to the amount of this tRNAArgspecies added to the reaction mixture. A mechanism for regulating the rate of protein synthesis at the translational level may be the slowing of polypeptide chain propagation at certain codons due to the presence of rate-limiting tRNA species.