A dual-genome Symbiosis Chip for coordinate study of signal exchange and development in a prokaryote-host interaction
A dual-genome Symbiosis Chip for coordinate study of signal exchange and development in a prokaryote-host interaction
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DOI：
10.1073/pnas.0407269101
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发表时间：
2004-11-23
影响因子：
11.1
通讯作者：
Long, SR
中科院分区：
文献类型：
--
作者：
Barnett, MJ;Tolman, CJ;Long, SR
生活在土壤中的α -变形杆菌Sinorhizobium meliloti与豆科植物进行共生：S. meliloti引发植物根瘤的形成，在那里它将二氮转化为氨供植物使用，以换取植物的光合作用。为了研究S. meliloti及其豆科伙伴在根瘤发育过程中的坐标分化，我们设计了一个定制的Affymetrix基因芯片，其中包含了S. meliloti完整的基因组和大约10000个探针集，用于植物宿主Medicago truncatula。用植物信号分子木草素培养的自由生活的S. meliloti在定义的最小和丰富的培养基中或在关键调节蛋白（NodD1， NodD3和RpoN）表达改变的菌株的表达谱证实了先前的数据并确定了先前未描述的调节靶点。对根瘤的分析表明，这种共生芯片可以同时研究双方的基因表达。我们的研究详细描述了共生关系中近5000个转录组的变化，并记录了不同环境下S. meliloti复杂的转录谱。
The soil-dwelling alpha-proteobacterium Sinorhizobium meliloti engages in a symbiosis with legumes: S. meliloti elicits the formation of plant root nodules where it converts dinitrogen to ammonia for use by the plant in exchange for plant photosynthate. To study the coordinate differentiation of S. meliloti and its legume partner during nodule development, we designed a custom Affymetrix GeneChip with the complete S. meliloti genome and approximate to10,000 probe sets for the plant host, Medicago truncatula. Expression profiling of free-living S. meliloti grown with the plant signal molecule luteolin in defined minimal and rich media or of strains altered in the expression of key regulatory proteins (NodD1, NodD3, and RpoN) confirms previous data and identifies previously undescribed regulatory targets. Analyses of root nodules show that this Symbiosis Chip allows the study of gene expression in both partners simultaneously. Our studies detail nearly 5,000 transcriptome changes in symbiosis and document complex transcriptional profiles of S. meliloti in different environments.