Influenza A virus specific T cell immunity in humans during aging
Influenza A virus specific T cell immunity in humans during aging
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DOI：
10.1006/viro.2002.1491
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发表时间：
2002-07-20
期刊：
影响因子：
3.7
通讯作者：
Osterhaus, ADME
中科院分区：
文献类型：
--
作者：
Boon, ACM;Fringuelli, E;Osterhaus, ADME
为了研究衰老过程中免疫系统的应答性降低，在18至70岁的健康献血者队列中研究了流感病毒特异性细胞免疫。通过流式细胞术测定甲型流感病毒特异性T细胞的百分比，发现其在老化过程中没有变化。在用佛波醇12-肉豆蔻酸酯13-乙酸酯和离子霉素刺激后，在衰老过程中观察到产生IFN-γ和IL-4的CD 8（+）T细胞的百分比增加。此外，在另外两组（18-20岁和68-70岁）5名供体中研究了细胞毒性T淋巴细胞（CTL）活性。在用甲型流感病毒体外刺激外周血单核细胞后，68- 70岁的供体中纯化的CD 8（+）T细胞的溶解能力似乎低于18- 20岁的供体。因此，我们的结论是，在老年人的CTL活性降低是不是一个较低的频率的病毒特异性T细胞的结果，但更有可能是受损的抗原特异性增殖或较低的裂解能力，这些细胞的结果。(C)2002 Elsevier Science（美国）。
To study the decreasing responsiveness of the immune system during aging, influenza virus specific cellular immunity was investigated in a cohort of healthy blood donors between 18 and 70 years of age. The percentage of influenza A virus specific T cells was determined by flow cytometry and found not to change during aging. After stimulation with phorbol 12-myristate 13-acetate and ionomycin, an increase in the percentage of IFN-gamma and IL-4 producing CD8(+) T cells was observed during aging. In addition, the cytotoxic T lymphocyte (CTL) activity was investigated in two additional groups of five donors, 18-20 and 68-70 years of age. The lytic capacity of purified CD8(+) T cells, after in vitro stimulation of peripheral blood mononuclear cells with influenza A virus, seemed lower in 68- to 70-year-old donors than in 18- to 20-year-old donors. Therefore we conclude that the reduced CTL activity in the elderly is not the result of a lower frequency of virus-specific T cells, but more likely the result of impaired antigen-specific proliferation or lower lytic capacity of these cells. (C) 2002 Elsevier Science (USA).