Production and uses of infectious in vitro sources of citrus tristeza virus
Production and uses of infectious in vitro sources of citrus tristeza virus
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柑橘tristeza病毒感染性体外来源的生产和用途
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
Moll
中科院分区：
文献类型：
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作者：
J. Garnsey;Muller;Moll
用柑桔衰退病毒（CTV）感染香橼的部分澄清和浓缩液感染BALB/C小鼠脾细胞，与小鼠骨髓瘤细胞系P3-X63-Ag8.653融合，制备杂交瘤细胞株。用来自加州的CTV株T514制备注射抗原。通过有限稀释法克隆杂交体，并筛选其对CTV感染和健康香橼汁液的反应性。反应组分的进一步表征将通过蛋白质平板凝胶电泳和蛋白质印迹分析进行。使用碱性磷酸酶缀合的亲和纯化的山羊抗小鼠IgG的固相ELISA进行克隆的菌株特异性分析。这些试验的结果表明，从我们的杂交瘤中可以获得菌株特异性或菌株区分性的单克隆抗体
Hybridomas were pre~ared by the fusion of mouse myeloma cells, cell line P3-X63-Ag8.653, and spleen cells from BALB/C mice nnrnunIzed with partially clarified and concentration sap from citrus tristeza virus (CTV) infected citron. A CTV strain, T514, from California was used for the preparation of inject antigen. Hybrids were cloned by limiting dilution and screened for reactivity to sap from CTV infected and healthy citron. Further characterization of the reacting components will be carried out by protein slab gel electrophoresis and western blot analysis. Analysis of clones for strain specificity was undertaken using a solid phase ELISA with alkaline phosphatase conjugated affinity purified goat anti-mouse IgG. Results of these tests indicate that strain specific or strain differentiating monoclonal antibodies might be obtained from our hybridomas