Immunohistochemical analysis of the pathogenicity of Helicobacter pylori infection:: Excess nitric oxide induced indirectly by Lewis X and Y is the cause of the pathogenicity of Helicobacter pylori infestation in the stomach
Immunohistochemical analysis of the pathogenicity of Helicobacter pylori infection:: Excess nitric oxide induced indirectly by Lewis X and Y is the cause of the pathogenicity of Helicobacter pylori infestation in the stomach
复制标题
DOI：
10.1267/ahc.35.93
复制
发表时间：
2002-01-01
影响因子：
2.4
通讯作者：
Murata, F
中科院分区：
文献类型：
--
作者：
Hasui, K;Nakamura, T;Murata, F
诱导型一氧化氮合酶（MOS）在幽门螺杆菌（HP）相关性溃疡患者胃粘膜相关淋巴组织（MALT）的生发中心（GC）和区域淋巴结（RLN）中表达。本研究采用免疫组化方法对4例MALT中iNOS的表达及MOS的诱导作用进行了研究。HP体表面粘膜和腺体中用抗HP、抗Lewis X和Y以及抗IgH抗体标记。刘易斯X或Y检测再生，化生和萎缩的腺上皮细胞和GCs的RLN。在MALT和RLN的部分腺上皮细胞和GC中再次识别到iNOS的表达。发生的MALT在普通粘膜免疫的背景下包括许多IgM（+）CD 5（-）细胞。1例IgM+ CD 5-细胞呈假结节状生长。提示HP小体的刘易斯X和Y可诱导GC中的iNOS。MALT中占优势的IgM+ CD 5-细胞表明，iNOS产生的过量一氧化氮（NO）干扰了GC中抗HP B细胞免疫的发育。由于NO也是一种诱变剂，IgM（+）CD 5-B细胞的结节状生长可能解释MALT型淋巴瘤的发生。
Inducible nitric oxide synthase (MOS) is expressed in the germinal centers (GCs) of mucosa-associated lymphoid tissue (MALT) and regional lymph nodes (RLNs) in the stomachs of patients with Helicobacter pylori (HP)-related ulcer. This study used immunohistochemistry to deduce how the iNOS was expressed and what the MOS induced on the MALT in four cases. HP bodies were labeled with anti-HP, anti-Lewis X and Y, and anti-IgH antibodies in the surface mucous coat and in the glands. Lewis X or Y was detected in regenerative, metaplastic and atrophic glandular epithelial cells and GCs of RLNs. The expression of iNOS was recognized again in some glandular epithelial cells and in the GCs of the MALT and RLNs. The developed MALT included many IgM(+) CD5(-) cells in the background of an ordinary mucosal immunity. Pseudonodular growth of the IgM+ CD5- cells was recognized in one case. It is suggested that Lewis X and Y from HP bodies induced iNOS in the GCs. Dominant IgM+ CD5- cells in the MALT suggested that excess nitric oxide (NO) produced by iNOS disturbed anti-HP B-cell immunity development in the GCs. As NO is also a mutagen, the IgM(+) CD5- B-cells' pseudonodular growth might explain MALT-type lymphomagenesis.