Activation of mitophagy in inflamed odontoblasts
Activation of mitophagy in inflamed odontoblasts
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发炎的成牙本质细胞中线粒体自噬的激活
DOI：
10.1111/odi.13110
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发表时间：
2019-09-01
期刊：
影响因子：
3.8
通讯作者：
Zhang, Lu
中科院分区：
文献类型：
--
作者：
Yang, Fuhua;Li, Yuan;Zhang, Lu
目的有丝分裂是线粒体质量控制的重要机制。在这项研究中，我们研究了炎性人牙髓和脂多糖刺激的前牙本质母细胞发生的线粒体损伤和有丝分裂。材料与方法用免疫荧光法和Western印迹法检测牙髓组织和脂多糖刺激的前牙本质母细胞中线粒体和有丝分裂相关蛋白的表达，并检测自噬标志物。线粒体产生的活性氧用MitoSOX法检测。用透射电子显微镜观察内毒素刺激的前牙本质母细胞线粒体的形态。结果炎症牙髓线粒体分裂活跃，有丝分裂现象明显。在脂多糖处理的成牙本质细胞中，吞噬丝裂相关蛋白也被上调。此外，炎症的前成牙本质细胞中的活性氧也增加。此外，在内毒素处理的成牙本质细胞中，还观察到含有部分降解的线粒体的单膜自溶体，内膜肿胀。结论炎症状态下成牙本质细胞线粒体受损，吞噬丝裂可能被激活，使受损的线粒体降解。
Objectives Mitophagy is an important mitochondrial quality control mechanism. In this study, we investigated the mitochondrial damage and mitophagy occurred in inflammatory human dental pulp and lipopolysaccharide-stimulated preodontoblasts. Materials and Methods In dental pulp tissues and lipopolysaccharide-stimulated preodontoblasts, immunofluorescences and Western blot were performed to detect the expression of mitochondrial and mitophagy-related proteins, and autophagy markers were also examined. Reactive oxygen species generated by mitochondria were examined by MitoSOX. Transmission electron microscope (TEM) was used to examine the morphology of mitochondria in lipopolysaccharide-stimulated preodontoblasts. Results The active fission activity of mitochondria and mitophagy in inflammatory dental pulp was observed. In lipopolysaccharide-treated preodontoblasts, mitophagy-related proteins were also upregulated. Moreover, increased reactive oxygen species in the inflamed preodontoblasts were observed. Additionally, single-membrane autolysosomes containing partially degraded mitochondria with swollen inner membranes in lipopolysaccharide-treated preodontoblasts were observed by TEM. Conclusions These results indicate that mitochondria were damaged and mitophagy might be activated to degrade impaired mitochondria in inflamed odontoblasts.