mRNA encoding 'CIC-K1, a kidney Cl--channel' is expressed in marginal cells of the stria vascularis of rat cochlea:: its possible contribution to Cl- currents
mRNA encoding 'CIC-K1, a kidney Cl--channel' is expressed in marginal cells of the stria vascularis of rat cochlea:: its possible contribution to Cl- currents
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DOI：
10.1016/s0304-3940(00)01021-1
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发表时间：
2000-04-28
影响因子：
2.5
通讯作者：
Takeuchi, S
中科院分区：
文献类型：
--
作者：
Ando, M;Takeuchi, S
耳蜗血管纹对毛细胞的正常功能至关重要，编码CIC-K1 Cl-通道的mRNA，以前被认为只在肾脏中发现，通过单细胞RT-PCR检测在大鼠血管纹上皮边缘细胞中。用全细胞膜片钳方法记录大鼠边缘细胞的Cl-电流，其稳态电流表现为较弱的外向整流，离子选择性顺序为SCN- > Br- = Cl- > F- > NO3- > I- > glucose（-）。Cl-电流的调节细胞外Ca 2+和pH值。这些特性类似于非洲爪蟾卵母细胞表达CIC-K1 Cl-通道记录的电流。这些数据共同表明，CIC-K1 Cl-通道可能有助于边缘细胞的全细胞电流。(C)2000爱思唯尔科学爱尔兰有限公司保留所有权利。
The cochlear stria vascularis is essential for the normal function of hair cells, mRNA encoding the CIC-K1 Cl- channel, previously thought to be found only in the kidney, was detected in epithelial marginal cells of the rat stria vascularis by single cell RT-PCR. When Cl- currents were recorded from rat marginal cells by the whole-cell patch clamp method, the steady-state currents showed weak outward rectification and an ion selectivity sequence of SCN- > Br- = Cl- > F- > NO3- > I- > gluconate(-). The Cl- currents were regulated by extracellular Ca2+ and pH. These characteristics resemble those reported for the currents recorded from Xenopus oocytes expressing CIC-K1 Cl- channels. These data together suggest that CIC-K1 Cl- channels may contribute to the whole-cell currents of marginal cells. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.