Coactosin accelerates cell dynamism by promoting actin polymerization
Coactosin accelerates cell dynamism by promoting actin polymerization
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DOI：
10.1016/j.ydbio.2013.04.006
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发表时间：
2013-07-01
影响因子：
2.7
通讯作者：
Nakamura, Harukazu
中科院分区：
文献类型：
--
作者：
Hou, Xubin;Katahira, Tatsuya;Nakamura, Harukazu
在发育过程中，细胞动态地移动或延伸它们的突起，这是通过肌动蛋白动力学实现的。在本研究中，我们注意到Coactosin，肌动蛋白结合蛋白，并研究其在肌动蛋白动力学中的作用。在神经嵴细胞和N1 E-115神经母细胞瘤细胞中，Coactosin与肌动蛋白和Capping蛋白相关。辅肌动蛋白在细胞过程中的积累及其与肌动蛋白丝的结合促使我们揭示辅肌动蛋白对细胞迁移的影响。在培养的神经嵴细胞中，辅肌动蛋白过度表达诱导细胞过程。相反，敲低辅肌动蛋白导致肌动蛋白聚合和神经嵴细胞迁移的中断。重要的是，辅肌动蛋白被招募到板状伪足和丝状伪足以响应Rac信号传导，并且不能与F-肌动蛋白结合的突变辅肌动蛋白不对Rac信号传导反应，也不支持神经嵴细胞迁移。研究还表明，显性负Rac 1（DN-Rac 1）剥夺Rac信号从神经嵴细胞干扰神经嵴细胞迁移，和DN-Rac 1和Coactosin的共转染恢复神经嵴细胞迁移。从这些结果中，我们得出结论，辅肌动蛋白的功能下游的Rac信号，它是参与神经突起的延伸和神经嵴细胞迁移，积极参与肌动蛋白聚合。(C)2013 Elsevier Inc. All rights reserved.
During development, cells dynamically move or extend their processes, which are achieved by actin dynamics. In the present study, we paid attention to Coactosin, an actin binding protein, and studied its role in actin dynamics. Coactosin was associated with actin and Capping protein in neural crest cells and N1E-115 neuroblastoma cells. Accumulation of Coactosin to cellular processes and its association with actin filaments prompted us to reveal the effect of Coactosin on cell migration. Coactosin overexpression induced cellular processes in cultured neural crest cells. In contrast, knock-down of Coactosin resulted in disruption of actin polymerization and of neural crest cell migration. Importantly, Coactosin was recruited to lamellipodia and filopodia in response to Rac signaling, and mutated Coactosin that cannot bind to F-actin did not react to Rac signaling, nor support neural crest cell migration. It was also shown that deprivation of Rac signaling from neural crest cells by dominant negative Rac1 (DN-Rac1) interfered with neural crest cell migration, and that co-transfection of DN-Rac1 and Coactosin restored neural crest cell migration. From these results we have concluded that Coactosin functions downstream of Rac signaling and that it is involved in neurite extension and neural crest cell migration by actively participating in actin polymerization. (C) 2013 Elsevier Inc. All rights reserved.