A MONOCLONAL-ANTIBODY TO AROMATIC AMINO-ACID HYDROXYLASES - IDENTIFICATION OF THE EPITOPE
A MONOCLONAL-ANTIBODY TO AROMATIC AMINO-ACID HYDROXYLASES - IDENTIFICATION OF THE EPITOPE
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DOI：
10.1042/bj2550193
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发表时间：
1988-10-01
影响因子：
4.1
通讯作者：
MORGAN, FJ
中科院分区：
文献类型：
--
作者：
COTTON, RGH;MCADAM, W;MORGAN, FJ
先前已经显示PH 8单克隆抗体与所有三种芳香族氨基酸羟化酶反应，特别适用于脑组织的免疫组织化学染色[哈安，詹宁斯，奎洛，中田，周，库辛斯基，布里廷汉姆和科顿（1987）脑研究426，19-27]。肝脏提取物的蛋白质印迹分析表明，PH 8反应与苯丙氨酸羟化酶从广泛的脊椎动物物种。抗体PH 8的表位已定位于氨基酸残基139和155之间的人苯丙氨酸羟化酶序列。芳香族氨基酸羟化酶的这个高度保守区域在苯丙氨酸羟化酶、酪氨酸羟化酶和色氨酸羟化酶中具有17个氨基酸中的11个相同。
PH8 monoclonal antibody has previously been shown to react with all three aromatic amino acid hydroxylases, being particularly useful for immunohistochemical staining of brain tissue [Haan, Jennings, Cuello, Nakata, Chow, Kushinsky, Brittingham and Cotton (1987) Brain Res. 426, 19-27]. Western-blot analysis of liver extracts showed that PH8 reacted with phenylalanine hydroxylase from a wide range of vertebrate species. The epitope for antibody PH8 has been localized to the human phenylalanine hydroxylase sequence between amino acid residues 139 and 155. This highly conserved region of the aromatic amino acid hydroxylases has 11 out of 17 amino acids identical in phenylalanine hydroxylase, tyrosine hydroxylase and tryptophan hydroxylase.