Suppression of viral replication by drs tumor suppressor via mTOR dependent pathway
Suppression of viral replication by drs tumor suppressor via mTOR dependent pathway
复制标题
drs肿瘤抑制因子通过mTOR依赖性途径抑制病毒复制
DOI：
10.1016/j.canlet.2011.09.015
复制
发表时间：
2012
期刊：
影响因子：
9.7
通讯作者：
et al
中科院分区：
文献类型：
--
作者：
Tambe;Y.;et al
drs基因是一种诱导肿瘤坏死的肿瘤抑制基因。通过使用drs基因敲除（KO）小鼠胚胎成纤维细胞（MEFs），我们发现drs参与宿主防御病毒感染。在drs-KO MEFs感染水泡性口炎病毒，病毒复制和蛋白质合成显着增强，而细胞蛋白质合成的上调。在drs-KO MEFs中，S6 K、S6、4 EBP 1和TSC 2蛋白的磷酸化与增强的病毒复制密切相关。Drs蛋白可与应激诱导的GADD 34结合形成与TSC 1/2的复合物，从而抑制mTOR活性。这些发现表明Drs通过mTOR依赖性途径抑制病毒复制。
The drs gene is an apoptosis-inducing tumor suppressor. By using drs-knockout (KO) mouse embryonic fibroblasts (MEFs), we showed that drs is involved in the host defense against viral infection. In drs-KO MEFs infected with vesicular stomatitis virus, the viral replication and protein synthesis were markedly enhanced without the upregulation of the cellular protein synthesis. Phosphorylation of S6K, S6, 4EBP1 and TSC2 proteins was closely correlated with the enhanced viral replication in drs-KO MEFs. Drs protein could associate with stress-inducible GADD34 to form a complex with TSC1/2, which suppresses mTOR activity. These findings indicate that Drs suppresses viral replication via mTOR-dependent pathway.