Probiotic Lactobacillus reuteri Ameliorates Disease Due to Enterohemorrhagic Escherichia coli in Germfree Mice
Probiotic Lactobacillus reuteri Ameliorates Disease Due to Enterohemorrhagic Escherichia coli in Germfree Mice
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DOI：
10.1128/iai.00880-10
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发表时间：
2011-01-01
影响因子：
3.1
通讯作者：
Britton, Robert A.
中科院分区：
文献类型：
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作者：
Eaton, Kathryn A.;Honkala, Alexander;Britton, Robert A.
肠出血性大肠杆菌（EHEC）菌株是一组产生滋贺毒素的食源性病原体，可引起严重的出血性结肠炎，并可导致溶血性尿毒症综合征（HUS），这是一种危及生命的疾病，主要影响儿童，目前尚无有效的治疗方法。我们使用无菌小鼠模型的肾脏和肠道疾病，由于肠出血性大肠杆菌，以确定是否益生菌罗伊氏乳杆菌ATCC PTA 6475是有效的抑制疾病症状引起的肠出血性大肠杆菌。当无菌瑞士韦伯斯特小鼠被肠出血性大肠杆菌单菌落感染时，它们会出现以体重减轻、盲肠腔液积聚和肾小管坏死为特征的疾病。当L.在EHEC攻击前1天给予reuteri，此后每隔一天给予一次，EHEC定殖受到抑制，小鼠明显免受疾病表现的影响。对疾病的保护并不需要在L.在治疗前，每日两次给药L.罗伊氏液似乎比每隔一天给药更有效。这些数据表明L. reuteri部分保护小鼠免受EHEC的疾病表现。
Strains of enterohemorrhagic Escherichia coli (EHEC) are a group of Shiga toxin-producing food-borne pathogens that cause severe hemorrhagic colitis and can lead to hemolytic-uremic syndrome (HUS), a life-threatening condition that principally affects children and for which there is no effective treatment. We used a germfree mouse model of renal and enteric disease due to EHEC to determine if probiotic Lactobacillus reuteri ATCC PTA 6475 is effective in suppressing disease symptoms caused by EHEC. When germfree Swiss Webster mice are monocolonized with EHEC, they develop disease characterized by weight loss, cecal luminal fluid accumulation, and renal tubular necrosis. When L. reuteri was administered 1 day prior to EHEC challenge and every other day thereafter, EHEC colonization was suppressed and mice were significantly protected from the manifestations of disease. Protection from disease did not require the induction of the antimicrobial compound reuterin in L. reuteri prior to treatment. The twice-daily administration of L. reuteri appeared more effective than every-other-day administration. These data indicated that L. reuteri partially protects mice from disease manifestations of EHEC.