Gut microbiota after gastric bypass in human obesity: increased richness and associations of bacterial genera with adipose tissue genes
Gut microbiota after gastric bypass in human obesity: increased richness and associations of bacterial genera with adipose tissue genes
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DOI：
10.3945/ajcn.113.058743
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发表时间：
2013-07-01
影响因子：
7.1
通讯作者：
Clement, Karine
中科院分区：
文献类型：
--
作者：
Kong, Ling-Chun;Tap, Julien;Clement, Karine
背景资料：Roux-en-Y胃旁路术（RYGB）是治疗病态肥胖症最有效的手术之一，可减轻体重，改善代谢和炎症。目的：我们研究了RYGB对肠道微生物群改变的影响及其与白色脂肪组织（WAT）基因表达变化的潜在关联。设计：在RYGB之前（0个月）、之后3个月和之后6个月，通过在病态肥胖个体中使用焦磷酸测序从粪便样品中分析肠道微生物群。在0和3个月时研究WAT基因表达。我们探讨了微生物属和差异表达的基因在WAT和临床markers.Results之间的关联：肠道菌群的丰富度增加后RYGB; 37%的增加的细菌属于变形菌。肠道微生物群组成和WAT基因表达之间的关联在RYGB后增加。RYGB后14个判别菌属（7个为优势菌属，7个为亚优势菌属）和202个WAT基因发生了变化。细菌属的变化与临床表型和脂肪组织基因表达的变化相关。一些基因编码代谢和炎症基因。几乎有一半的相关性是独立的变化，在热量intake.Conclusion：这些结果表明增加肠道菌群的丰富性和肠道菌群和WAT基因之间的关联后RYGB在肥胖症的数量。肠道微生物群的变化与WAT基因表达的变化相关。这些发现激发了对人类肥胖及其减肥后变化的肠道微生物组和WAT病理改变之间联系机制的更深入探索。
Background: Roux-en-Y gastric bypass (RYGB) surgery is one of the most efficient procedures for treating morbid obesity and results in weight-loss and improvements in metabolism and inflammation.Objective: We examined the impact of RYGB on modifications of gut microbiota and its potential associations with changes in gene expression in white adipose tissue (WAT).Design: Gut microbiota were profiled from fecal samples by using pyrosequencing in morbidly obese individuals, explored before (0 mo), 3 mo after, and 6 mo after RYGB. WAT gene expression was studied at 0 and 3 mo. We explored associations between microbial genera and differentially expressed genes in WAT and clinical markers.Results: The richness of gut microbiota increased after RYGB; 37% of increased bacteria belonged to Proteobacteria. The associations between gut microbiota composition and WAT gene expression increased after RYGB. Fourteen discriminant bacterial genera (7 were dominant and 7 were subdominant) and 202 WAT genes changed after RYGB. Variations in bacterial genera correlated with changes in both clinical phenotype and adipose tissue gene expression. Some genes encode metabolic and inflammatory genes. Almost half of the correlations were independent of the change in calorie intake.Conclusion: These results show an increase in gut microbiota richness and in the number of associations between gut microbiota and WAT genes after RYGB in obesity. Variations of gut microbiota were associated with changes in WAT gene expression. These findings stimulate deeper explorations of the mechanisms linking gut microbiome and WAT pathological alterations in human obesity and its changes after weight loss.