Effects of AG490 on lens epithelial cell death induced by H2O2
Effects of AG490 on lens epithelial cell death induced by H2O2
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DOI：
10.1007/s10384-009-0772-6
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发表时间：
2010-03-01
影响因子：
2.4
通讯作者：
Lee, Joon H.
中科院分区：
文献类型：
--
作者：
Kim, Ungsoo Samuel;Nam, Sang-Min;Lee, Joon H.
To evaluate the influence of H2O2 on lens epithelial cells (LECs) and to determine the effect of the Janus kinase (JAK) inhibitor AG490 and the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase (MEK) inhibitor U0126 on LEC death after H2O2 exposure.Human lens epithelial (HLE) B-3 cells were cultured. Cells were treated for 45 min with H2O2 and signal transducers and activators transcription (STAT) 3, JAK2, and ERK1/2 phosphorylation were surveyed by Western blot analysis. After pretreatment with either 40 mu M AG490 or 25 mu M U0126, LECs were exposed to H2O2. LEC death was evaluated by microscopy and flow cytometry.H2O2 induced phosphorylation of Tyr-705 STAT3, JAK2, and ERK1/2 in LECs. In cells pretreated with both AG490 and U0126, phosphorylation of Tyr-705 STAT3, JAK2, and ERK1/2 was suppressed. Microscopic findings, however, showed that only AG490 noticeably enhanced cell survival, and flow cytometry showed that cell necrosis decreased to 4.05% after pretreatment with 40 mu M AG490.AG490 may prevent H2O2-induced LEC death by blocking an unknown necrosis pathway. Further investigation is required to characterize that pathway.
